FITC (Fluorescein Isothiocyanate)-conjugated anti-guinea pig antibodies are fluorescent immunoreagents designed for the direct detection of guinea pig antigens in a wide range of immunological applications. FITC is one of the most extensively used fluorophores for antibody labeling, producing bright green fluorescence with an excitation maximum of approximately 493 nm and an emission maximum of approximately 517 nm, making it fully compatible with standard 488 nm laser-based instruments.

Formats and Specificity

These antibodies are available as whole IgG or Fab fragments to accommodate different experimental requirements. They typically recognize guinea pig IgG heavy and light chains (H+L), while cross-adsorbed formats help minimize cross-reactivity with immunoglobulins from other species. Antibody specificity is routinely verified using immunoelectrophoresis and ELISA, ensuring reliable and reproducible antigen detection.

Applications

• Immunofluorescence: Enables direct visualization of guinea pig antigens with bright green fluorescence, typically used at dilutions ranging from 1:100 to 1:250.
• Flow cytometry and immunocytochemistry: Validated for sensitive fluorescent detection, with recommended working dilutions generally between 1:500 and 1:20,000 depending on the assay conditions.
• Fab fragment applications: Ideal for direct immunofluorescence and indirect ELISA, reducing Fc-mediated interactions and minimizing non-specific binding.

Storage

FITC-conjugated antibodies are commonly supplied in phosphate-buffered saline (PBS) containing bovine serum albumin (BSA) and sodium azide as a preservative. For optimal stability, store at 4°C for short-term use or at −20°C for long-term storage in the presence of glycerol. Protect the conjugates from prolonged light exposure and avoid repeated freeze-thaw cycles to preserve fluorescence intensity and antibody performance.