PE/Atto 594-conjugated anti-chicken primary antibodies are affinity-purified antibodies directed against chicken (Gallus gallus) immunoglobulins or specific chicken proteins and conjugated to a dual-label system combining R-phycoerythrin (PE) and Atto 594. This dual labeling provides a highly flexible reagent: the PE moiety supplies exceptional brightness for flow cytometry and sorting, while Atto 594 grants a spectrally distinct, photostable red fluorescence for imaging applications. Dual-conjugated primaries support multimodal workflows (FACS + microscopy), validation across platforms, and improved multiplexing options.
Key features
- Dual fluorophore labeling: Covalent conjugation to both PE (high brightness) and Atto 594 (photostable red dye) on the same antibody molecule.
- High performance: Optimized dye:protein (D/P) ratios to preserve antigen affinity and ensure robust fluorescence from both labels.
- Affinity purified: Monoclonal and polyclonal formats available to reduce nonspecific binding.
- Multimodal utility: Validated for flow cytometry, confocal and widefield fluorescence microscopy, immunofluorescence, and plate-based assays.
- Stabilized formulations: Supplied in buffers with stabilizers (e.g., BSA) and preservative options (azide-free available for live-cell work).
- Lot QC: Each lot characterized for D/P ratios (PE and Atto 594), percent purity, fluorescence intensity (MFI), and functional performance.
Common applications
- Flow cytometry and cell sorting: PE provides bright signal and sensitivity for detection and sorting of rare populations.
- Fluorescence microscopy and confocal imaging: Atto 594 used for high-resolution imaging with good photostability and compatibility with red emission filters.
- Correlative experiments: Same antibody enables FACS-based quantification (PE) and imaging-based localization (Atto 594) ensuring consistent antigen recognition.
- Multicolor panels: PE/Atto 594 can occupy separate channels in complex panels or provide confirmatory readouts across platforms.
- Bead-based and plate assays: Flexible readouts via flow or fluorescent plate readers using appropriate excitation/emission settings.