Library quantifcation master mix for Illumina®

Accurate quantitation of the number of molecules in a library is the most critical step in the preparation steps for next-generation sequencing (NGS) to obtain high-quality reading data. Indeed, in order to obtain reliable and quality reading data, it is necessary to use a sufficient quantity of DNA, and in particular for Illumina® sequencing. The use of insufficient DNA will result in low sequencing efficiency and DNA overabundance will increase cluster density and result in poor quality data.

Quantitative PCR is considered to be the most accurate and efficient method of quantification of NGS library.

The library quantitation kits for Illumina® sequencing contain all the components bellow:

DNA polymerase
dNTPs
MgCl2
Primers to amplify only the molecules that contain the two sequences of adapters
SYBR green or EvaGreen
A ROX solution to add depending on the device used. If the ROX solution is separate from the mix, it is a universal kit that can be used with all instruments. If ROX is contain in the master mix, it is necessary to use the appropriate kits with your device.

Universal	All instruments
Without ROX	Bio-Rad CFX96, CFX384, iCycler iQ, iQ5, MyiQ, MiniOpticon, Opticon®, Opticon 2, Chromo4; Cepheid SmartCycler®; Eppendorf Mastercycler® ep realplex, realplex 2 s; Illumina Eco qPCR; Qiagen/Corbett Rotor-Gene® Q, Rotor-Gene® 3000, Rotor-Gene® 6000; Roche Applied Science LightCyclerTM 480; Thermo Scientific PikoReal Cycler.
Low ROX	Applied Biosystems 7500, 7500 Fast, ViiA7; Stratagene MX4000, MX3005P, MX3000P
High ROX	Applied Biosystems 5700, 7000, 7300, 7700, 7900, 7900HT, 7900HT Fast; StepOne, StepOnePlus

Frequently Asked Questions (FAQ)

How can I quickly find a master mix product?

To find a master mix product, use the search function by name, product code, supplier, brand or sub-category. You can also browse the product families listed on the page to identify an available product code, an alternative or an equivalent product.

Does Biovalley stock several product codes in the master mix category?

Yes, Biovalley distributes several product references in the master mix category, sourced from specialist suppliers. Depending on the product family, references may vary in terms of format, packaging, brand, supplier, technical specifications or availability.

Can I request a quote for master mix products?

Yes, you can request a quote for master mix products from the product page or by contacting Biovalley (info@biovalley.fr). To help us process your enquiry, please provide, where possible, the product name, catalogue reference, required quantity, the supplier or brand you are looking for, and the country of delivery.

How can I find out if a master mix product is available to order?

The availability of a master mix product depends on the product code, supplier, packaging and country of delivery. For up-to-date information, please check the product details page or contact Biovalley with the product code or name of the product you are looking for.

What should I do if I cannot find the master mix product I am looking for?

If the master mix product you are looking for does not appear in the catalogue, please contact Biovalley with the available information: product name, reference number, supplier, brand, format or packaging. The team will be able to search for the reference number within its network of suppliers or suggest an available alternative.

Are all master mix products available under the same terms and conditions?

No. Ordering, delivery, storage and documentation conditions may vary depending on the product type, the supplier and the applicable regulations. Certain product codes may require specific approval, specialised transport, a safety data sheet or additional information prior to ordering.