Detergents play a crucial role in various biological and biochemical processes, including the purification and classification of lipid rafts. Triton , a widely used detergent, has been employed for the purification of rafts, along with other detergents such as CHAPS, Triton X-114, Lubrol WX, Brij96, and Brij98. These detergents have been utilized to solubilize specific components of the inner plasma membrane leaflet, with Triton being more effective at solubilizing certain components than other detergents. Additionally, Triton X-100 has been used in the optimization and in silico analysis of cold-adapted lipase from Antarctic Pseudomonas sp. strain AMS8 reaction in reverse micelles, demonstrating its diverse applications. Furthermore, Triton has been studied in the context of observing the solubilization of lipid bilayers by detergents with optical microscopy of giant unilamellar vesicles (GUVs). It has also been compared with other non-ionic detergents, such as Tween-20, for its effectiveness in mitigating the reduction in cerebrospinal fluid o-α-synuclein levels.

Moreover, Triton has been associated with the formation of detergent-resistant membranes (DRMs) or detergent-insoluble membranes (DIMs), which are buoyant membrane fractions that form in the presence of certain nonionic detergents at low temperatures. This highlights the significance of Triton in studying membrane subdomains and their resistance to detergent disruption. Additionally, Triton has been used in the recovery of recombinant cutinase and in the solubilization of the fat globule membrane of bovine milk, showcasing its relevance in biotechnological applications.

Triton is a versatile detergent with diverse applications in lipid raft purification, solubilization of membrane components, and various biotechnological processes. Its effectiveness in solubilizing specific membrane components and its role in studying membrane subdomains make it a valuable tool in biological and biochemical research.