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> 4E Binding Protein 1, phosphorylated (Ser65) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

4E Binding Protein 1, phosphorylated (Ser65) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

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Cat# 0004-20-100ul

Size : 100ul

Brand : US Biological

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Phone : +1 850 650 7790

0004-204E Binding Protein 1, phosphorylated (Ser65) (Protein Synthesis Initiation Factor 4E Binding Protein, eIF-4EBP1, PHAS-1, PHAS-I, Eukaryotic Translation Initiation Factor BP)

Specifications
Clone TypePolyclonal
Hostrabbit
Sourcemouse
Swiss ProtQ60876
IsotypeIgG
GradeAffinity Purified
ApplicationsE IP WB
CrossreactivityHu Mk Mo Rt
Shipping TempBlue Ice
Storage Temp-20°C

4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyperphosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues; phosphorylation by FRAP/mTOR on Thr37 and Thr46 of human 4E-BP1 may prime it for subsequent phosphorylation at sites including Ser65 and Thr70.

Applications:
Suitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight
Immunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: mouse|Concentration: Not Determined|Form: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.|Purity: Purified by Protein A and peptide affinity chromatography.|Immunogen: Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.|Specificity: Recognizes endogenous levels of mouse 4E-BP1 only when phosphorylated at Ser65. Has also been shown to recognize 4E-BP1 when phosphorylated at serine 101. Species Crossreactivity: human, rat and monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.
Purity
Purified by Protein A and peptide affinity chromatography.
Specificity
Recognizes endogenous levels of mouse 4E-BP1 only when phosphorylated at Ser65. Has also been shown to recognize 4E-BP1 when phosphorylated at serine 101. Species Crossreactivity: human, rat and monkey.
References
1. Pause, A. et al. (1994) Nature 371, 762–767. 2. Brunn, G.J. et al. (1997) Science 277, 99–101. 3. Gingras, A.C. et al. (1998) Genes Dev. 12, 502–513. 4. Fadden, P. et al. (1997) J. Biol. Chem. 272, 10240–10247. 5. Gingras, A.C. et al. (1999) Genes Dev. 13, 1422–1437.