GTX629765 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] in MeDIP experiment.
Human genomic DNA (500 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) at dilution of 1:500 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX629765 5-hydroxymethylcytosine antibody [GT13612] Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX629765) at a dilution of 1:500.
A: Unmethylated DNA fragment
B: DNA fragment containing 5-methylcytosine
C: DNA fragment containing 5-hydroxymethylcytosine
D: DNA fragment containing 5-formylcytosine
E: DNA fragment containing 5-carboxylcytosine

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] detects 5-HydroxymethylCytidine protein at nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
5-Hydroxymethylcytosine / 5-hmC antibody [GT13612] (GTX629765) dilution: 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min