A549/GFP Cell Line
Cat# MBS168483-5x1
Size : 5x1Vial
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
A549/GFP Cell Line
Figure 1. A549/GFP Cell Line. Left: GFP Fluorescence; Right: Phase Contrast
A549/GFP Cell Line
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS168483Background: The A549 cell line was first developed in 1972 through the removal and culturing of cancerous lung tissue in the explanted tumor of 58-year-old male. A549 cells are human alveolar basal epithelial cells. They are squamous in nature and responsible… More details
Product Overview
Online Datasheet in PDFProduct Name
A549/GFP, Cell Line
Full Product Name
A549/GFP Cell Line
Product Synonym Names
A549/GFP Cell Line
Concentration
1 mL, 1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO (lot specific)
Preparation and Storage
Liquid nitrogen
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80 degrees C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Note: For best results begin culture of cells immediately upon receipt. If this is not possible, store at -80 degrees C until first culture. Store subsequent cultured cells long term in liquid nitrogen.
Shipping Note: Product is available for shipment in the United States, Canada and European countries. Please inquire for shipment to other countries.
Quality Control
This cryovial contains at least 1.0 x 106 A549/GFP cells as determined by morphology, trypan-blue dye exclusion, and viable cell count. The A549/GFP cells are tested free of microbial contamination.
Medium
1. Culture Medium: D-MEM (high glucose), 10% fetal bovine serum (FBS), 0.1 mM MEM Non-Essential Amino Acids (NEAA), 2 mM L-glutamine, 1% Pen-Strep.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
2. Freeze Medium: 70% DMEM, 20% FBS, 10% DMSO.
Methods
Establishing A549/GFP Cultures from Frozen Cells
1. Place 10 mL of complete DMEM or McCoy's 5A growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37 degrees C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37 degrees C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
1. Place 10 mL of complete DMEM or McCoy's 5A growth medium in a 50-mL conical tube. Thaw the frozen cryovial of cells within 1-2 minutes by gentle agitation in a 37 degrees C water bath. Decontaminate the cryovial by wiping the surface of the vial with 70% (v/v) ethanol.
2. Transfer the thawed cell suspension to the conical tube containing 10 ml of growth medium.
3. Collect the cells by centrifugation at 1000 rpm for 5 minutes at room temperature. Remove the growth medium by aspiration.
4. Resuspend the cells in the conical tube in 15 mL of fresh growth medium by gently pipetting up and down.
5. Transfer the 15 mL of cell suspension to a T-75 tissue culture flask. Place the cells in a 37 degrees C incubator at 5% CO2.
6. Monitor cell density daily. Cells should be passaged when the culture reaches 95% confluence.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Product Categories/Family for A549/GFP cell line
Cell Signaling and Protein Biology; Reporter Assays; Cells and Reagents; Reporter Stable Cell Lines
Other Notes
Small volumes of A549/GFP cell line vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for A549/GFP purchase
MBS168483 is a cell line targeting A549/GFP.