Adenosine Diphosphate (ADP) BioAssay™ ELISA Kit (General)

Sample Type:
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Intended Use:
The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of ADP in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Sensitivity:
7.63ng/ml

Range:
18.52-1500ng/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of Adenosine Diphosphate (ADP). No significant cross-reactivity or interference between Adenosine Diphosphate (ADP) and analogues was observed.

Precision:
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Test Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to ADP has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled ADP and unlabeled ADP (Standards or samples) with the pre-coated antibody specific to ADP. After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of ADP in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of ADP in the sample.

Kit Components:
96-well strip plate, 1x Plate
Standard, 2x vial
Detection Reagent A, 1
Detection Reagent B, 1×120ul
Reagent Diluent, 1×300ul
TMB Substrate, 1×9ml
Wash Buffer (30X), 1×20ml
Standard Diluent, 1×20ml
Assay Diluent A, 1×12ml
Assay Diluent B, 1×12ml
Stop Solution, 1x6ml

Storage and Stability:
Store all components at 4°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.