AEC Peroxidase Chromogen Substrate Kit
Cat# MBS258523-400mL
Size : 400mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
AEC Reagent
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258523Peroxidase chromogen-substrate 3-amino-9-ethyl-carbazole (AEC) is an excellent chromogen system that is used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This chromogen system gives bright red color and is very compatible with hematoxylin… More details
Product Overview
Online Datasheet in PDFProduct Name
AEC Peroxidase Chromogen, Reagent
Full Product Name
AEC Peroxidase Chromogen Substrate Kit
Product Gene Name
AEC reagent
Preparation and Storage
Store at 2-8 degree C
Kit Contents
Reagent B
Concentrated Buffer: 5 ml
Reagent C: Chromogen:
2.5 ml
Reagent S: Substrate: 3 ml
Concentrated Buffer: 5 ml
Reagent C: Chromogen:
2.5 ml
Reagent S: Substrate: 3 ml
Product Categories/Family for AEC reagent
IHC Reagents
Applications Tested/Suitable for AEC reagent
Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes for AEC reagent
(THIS KIT CONTAINS approximately 166 X CHROMOGEN, When diluted will make approx. 425 ml))
1. To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well.
2. Add one drop of reagent C chromogen, mix well.
3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country's regulations.
Application: IHC, ICC
1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/deionized water.
2. Add few drops of ready to use AEC reagent, incubate at RT or 37 degree C for 5-10 minutes.
3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
4. Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds .
5. Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
6. Mount slide with aqueous mounting medium, we use our IHM please follow the protocol for mounting medium.
1. To 5 ml of distilled or deionized water in a test tube, add two drop of reagent B buffer, mix well.
2. Add one drop of reagent C chromogen, mix well.
3. Add one drop of reagent S substrate, mix well. This ready to use reagent is good for several hours. The unused AEC solution can be discarded according to city, county, state, province or country's regulations.
Application: IHC, ICC
1. After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated), and distilled/deionized water.
2. Add few drops of ready to use AEC reagent, incubate at RT or 37 degree C for 5-10 minutes.
3. Wash 2-5X with buffer, followed by rinsing with distilled/deionized water.
4. Add counterstain compatible with AEC, we use hematoxylin 30-60 seconds .
5. Wash with tap water, buffer pH 7.4 or higher followed by rinsing with distilled/deionized water.
6. Mount slide with aqueous mounting medium, we use our IHM please follow the protocol for mounting medium.
Other Notes
Small volumes of AEC reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for AEC purchase
MBS258523 is a reagent targeting AEC. Also known as AEC Peroxidase Chromogen Substrate Kit.