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> Anti-iNOS Antibody

Anti-iNOS Antibody

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Cat# CPA4955-100ul

Size : 100ul

Brand : Cohesion Biosciences

Contact local distributor :


Phone : +1 850 650 7790

  • Western blot analysis of iNOS expression in BV2 (A), LO2 (B), HepG2 (C) whole cell lysates. (Predicted band size: 131 kD; Observed band size: 131 kD)
  • Immunohistochemical analysis of iNOS staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Immunofluorescent analysis of iNOS staining in SVHUC1 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Product Name:Anti-iNOS Antibody
Cat No:CPA4955
Source:Rabbit
Reactivity:H, M, R, B
Applications:WB, IH, IF/IC
*Application Key:
H - Human, M - Mouse, R - Rat, B - Bovine, C - Chicken, D - Dog, G - Goat, Mk - Monkey, P - Pig, Rb - Rabbit, S - Sheep, Z - Zebrafish
*Species Reactivity Key:
E- ELISA, WB - Western blot, IH - Immunohistochemistry, IF - Immunofluorescence, FC - Flow cytometry, IC - Immunocytochemistry, IP - Immunoprecipitation, ChIP - Chromatin Immunoprecipitation, EMSA - Electrophoretic Mobility Shift Assay, BL - Blocking, SE - Sandwich ELISA, CBE - Cell-based ELISA, RNAi - RNA interference
Description：Rabbit polyclonal antibody to iNOS
Immunogen：KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human iNOS. The exact sequence is proprietary.
Purification：The antibody was purified by immunogen affinity chromatography.
Clonality：Polyclonal
Form：Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Dilution：WB (1/500 - 1/1000), IH (1/100 - 1/200), IF/IC (1/50 - 1/200)
Gene Symbol：NOS2
Alternative Names：NOS2A; Nitric oxide synthase, inducible; Hepatocyte NOS; HEP-NOS; Inducible NO synthase; Inducible NOS; iNOS; NOS type II; Peptidyl-cysteine S-nitrosylase NOS2
Entrez Gene (Human)： 4843;
Entrez Gene (Mouse)： 18126;
Entrez Gene (Rat)： 24599;
SwissProt (Human)： P35228;
SwissProt (Mouse)： P29477;
SwissProt (Rat)： Q06518;
Storage/Stability：Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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