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Reagents and instruments for immunology, cell biology and molecular biology.

Anti-Talin (Rod domain) Antibody

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Cat# TM4081

Size : 100uL

Contact local distributor :

Phone : +1 850 650 7790

Brand : Phosphosolutions

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Contact local distributor :

Phone : +1 850 650 7790

Anti-Talin (Rod domain) Antibody (8D4)

Our Talin (Rod domain) primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, rat, and Xenopus Talin (Rod domain) and is Protein A purified. It is great for use in WB, ICC, IP.

Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.

Immunocytochemical labeling of Talin relative to F-actin in chick fibroblasts. The cells were labeled with mouse monoclonal Talin (Rod domain) antibody (TM4081), then the antibody was detected using appropriate secondary antibody (Red). This labeling is compared to F-actin staining (Green). (Image provided by Dr. Gianluca Gallo at Drexel University).

Western blot of rat PC12 cells stimulated with Calyculin A (100 nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.

Click on image to zoom

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Applications

ICC, IP, WB

Host Species

Mouse

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SKU: TM4081

Ships: 1-2 business days

Product Details

Target

Talin (Rod domain)

Scientific Background

Talin is an important cytoskeletal component of integrin adhesion sites. Calpins cleave talin precursor (240 kDa) into an amino-terminal globular head domain of 47 kDa and a carboxyl-terminal 190 kDa rod domain. The talin head domain contains a FERM domain that binds integrins, PIP kinase (Type I), and FAK. The rod domain has several vinculin-binding sites, a second integrin-binding site, and two actin-binding sites. These talin protein-protein interactions are critical for integrin activation, focal adhesion formation, and cell migration. Talin regulation may occur through phosphorylation and regulated degradation. The talin head domain binds Smurf1, an E3 ubiquitin ligase, and this interaction leads to talin head ubiquitylation and degradation. Cdk5 can phosphorylate Ser-425 in the head domain, and this inhibits both binding to Smurf1 and subsequent degradation. The S425A talin mutant resists Cdk5 phosphorylation, increases susceptibility to Smurf1-mediated ubiquitylation, and inhibits cell migration. Thus, talin head phosphorylation may be important for regulating adhesion stability and cell migration

Format

Protein A Purified

Clonality

Monoclonal

Clone

8D4

Isotype

IgG1

Applications

ICC, IP, WB

Host Species

Mouse

Gene Name

TLN1

Molecular Weight

240

Immunogen

Clone 8D4 was generated from purified chicken gizzard talin. The antibody recognizes an epitope within the rod domain of talin from many species, including human, rat, mouse, chicken, and frog.

Immunogen Species

Chicken

Species Reactivity

Chicken, Frog, Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000
ICC: 1:100
IP: 1:100

Conjugate

Unconjugated

Specificity

The antibody detects a 240 kDa* protein corresponding to the molecular mass of Talin on SDS-PAGE immunoblots of human A431, rat PC12, and rabbit fibroblast cells.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

TLN1

UniProt ID

P54939

Shipping

Blue Ice

Anti-Talin (Rod domain) Antibody

Cat# TM4081

Contact local distributor :

Brand : Phosphosolutions

Contact local distributor :

Anti-Talin (Rod domain) Antibody (8D4)

SKU: TM4081

Product Details

Bulk Order Anti-Talin (Rod domain) Antibody