Reagents and instruments for immunology, cell biology and molecular biology.
 
> ATM (A-T Mutated, A-T Mutated Homolog, AT Complementation Group A, AT Complementation Group C, AT Complementation Group D, AT Complementation Group E, AT Mutated, AT Protein, AT1, ATA, Ataxia Telangiectasia Gene Mutated in Human Beings, Ataxia Telangiectasia Mutated, Ataxia Telangiectasia Mutated Gene, Ataxia Telangiectasia Mutated Homolog (Human), Ataxia Telangiectasia Mutated Homolog, ATC, ATD, ATDC, ATE, ATM Serine/Threonine Kinase, ATM_HUMAN, Human Phosphatidylinositol 3 Kinase Homolog, Serine Protein Kinase ATM, Serine-protein Kinase ATM, Serine/threonine-protein Kinase ATM, T Cell Prolymphocytic Leukemia, T Cell Prolymphocytic Leukemia, Tefu, TEL1, Telomere Maintenance 1 Homolog, TELO1, Telomere Fusion Protein, TPLL)

ATM (A-T Mutated, A-T Mutated Homolog, AT Complementation Group A, AT Complementation Group C, AT Complementation Group D, AT Complementation Group E, AT Mutated, AT Protein, AT1, ATA, Ataxia Telangiectasia Gene Mutated in Human Beings, Ataxia Telangiectasia Mutated, Ataxia Telangiectasia Mutated Gene, Ataxia Telangiectasia Mutated Homolog (Human), Ataxia Telangiectasia Mutated Homolog, ATC, ATD, ATDC, ATE, ATM Serine/Threonine Kinase, ATM_HUMAN, Human Phosphatidylinositol 3 Kinase Homolog, Serine Protein Kinase ATM, Serine-protein Kinase ATM, Serine/threonine-protein Kinase ATM, T Cell Prolymphocytic Leukemia, T Cell Prolymphocytic Leukemia, Tefu, TEL1, Telomere Maintenance 1 Homolog, TELO1, Telomere Fusion Protein, TPLL)

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Cat# 303137-100ug

Size : 100ug

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

303137 ATM (A-T Mutated, A-T Mutated Homolog, AT Complementation Group A, AT Complementation Group C, AT Complementation Group D, AT Complementation Group E, AT Mutated, AT Protein, AT1, ATA, Ataxia Telangiectasia Gene Mutated in Human Beings, Ataxia Telangiectasia Mutated, Ataxia Telangiectasia Mutated Gene, Ataxia Telangiectasia Mutated Homolog (Human), Ataxia Telangiectasia Mutated Homolog, ATC, ATD, ATDC, ATE, ATM Serine/Threonine Kinase, ATM_HUMAN, Human Phosphatidylinositol 3 Kinase Homolog, Serine Protein Kinase ATM, Serine-protein Kinase ATM, Serine/threonine-protein Kinase ATM, T Cell Prolymphocytic Leukemia, T Cell Prolymphocytic Leukemia, Tefu, TEL1, Telomere Maintenance 1 Homolog, TELO1, Telomere Fusion Protein, TPLL)

Clone Type
Polyclonal
Host
rabbit
Source
human
Swiss Prot
Q13315
Isotype
IgG
Grade
Affinity Purified
Applications
WB
Crossreactivity
Hu
Shipping Temp
Blue Ice
Storage Temp
-20°C

ATM(ataxia telangiectasia mutated), also known as TEL1 or TELO1, is a serine/threonine protein kinase that is recruited and activated by DNA double-strand breaks. The ATM protein is a member of the phosphatidylinositol 3-kinase family of proteins that respond to DNA damage by phosphorylating key substrates involved in DNA repair and/or cell cycle control. The ATM gene is mapped to chromosome 11q22.3. ATM has an essential role in the reconstitutive capacity of hematopoietic stem cells but is not as important for the proliferation or differentiation of progenitors in a telomere-independent manner. ATM functions directly in the repair of chromosomal DNA double-stranded breaks by maintaining DNA ends in repair complexes generated during lymphocyte gene assembly.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.5ug/ml, detection limit is ~0.25ng/lane under reducing conditions.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Applications
Product Type: Pab|Isotype: IgG|Host: rabbit|Source: human|Concentration: ~0.5mg/ml (after reconstitution)|Form: Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.|Purity: Purified by immunoaffinity chromatography.|Immunogen: Recombinant protein corresponding to human ATM expressed in E. coli. (Position: D2870-V3056). Species sequence homology: mouse (95%)|Specificity: Recognizes human ATM. ||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Recombinant protein corresponding to human ATM expressed in E. coli. (Position: D2870-V3056). Species sequence homology: mouse (95%)
Form
Supplied as a lyophilized powder from PBS, 5% BSA, 0.05% sodium azide. Reconstitute with 200ul sterile dH2O.
Purity
Purified by immunoaffinity chromatography.
Specificity
Recognizes human ATM.
References
1. Allen, D. M., van Praag, H., Ray, J., Weaver, Z., Winrow, C. J., Carter, T. A., Braquet, R., Harrington, E., Ried, T., Brown, K. D., Gage, F. H., Barlow, C. Ataxia telangiectasia mutated is essential during adult neurogenesis. Genes Dev. 15: 554-566, 2001. 2. Bakkenist, C. J., Kastan, M. B. DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation.Nature 421: 499-506, 2003. 3. Chong, M. J., Murray, M. R., Gosink, E. C., Russell, H. R. C., Srinivasan, A., Kapsetaki, M., Korsmeyer, S. J., McKinnon, P. J. Atm and Bax cooperate in ionizing radiation-induced apoptosis in the central nervous system. Proc. Nat. Acad. Sci. 97: 889-894, 2000.