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> B Cell Subset (PE)

B Cell Subset (PE)

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Cat# B0001-65C-100ug

Size : 100ug

Brand : US Biological

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Phone : +1 850 650 7790

B0001-65CB Cell Subset (PE)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourceporcine
IsotypeIgM,k
GradePurified
ApplicationsFC IHC
CrossreactivityPo
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

While identification of the antigen(s) that are found predominantly on ILPP B cells and which are recognized by this antibody is speculative, the antibody appears to react with an immature population of B cells in the follicles of ILPPs and with a subpopulation of sIglow B cells in lymph nodes.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen sections (acetone fixed).
Flow Cytometry: ≤ 0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Applications
Product Type: Mab|Isotype: IgM,k|Clone No: BB6-10A10|Host: mouse|Source: porcine|Concentration: ~0.1mg/ml|Form: Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.|Purity: Purified|Specificity: Recognizes porcine B cell subset. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Form
Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with PE.
Purity
Purified
Specificity
Recognizes porcine B cell subset. Stains the following percentages of surface immunoglobulin (sIg) positive cells in porcine lymphoid tissues: 11-15% in peripheral blood; 22-23% in mesenteric lymph nodes; 79-87% in ileal Peyer’s patches (ILPP); 6-17 in spleen; and 0% in thymus.
References
1. Denham, S., R.J. Zwart, J.T.D. Whittall, M. Pampusch, A.H. Corteyn, A.T.J. Bianchi, M.P. Murtaugh, R.M.E. Parkhouse, H. Tlaskalova, J. Sinkora, M. Siinkora, and Z. Rehakova. 1998. Monoclonal antibodies putatively identifying porcine B cells. Veter. Immunol. Immunopathol. 60:317-328.