BCIP/NBT Substrate
Cat# MBS539295-100mL
Size : 100mL
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
BCIP/NBT Substrate
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS539295MBS539295 is a substrate targeting BCIP/NBT Substrate. More details
Product Overview
Online Datasheet in PDFProduct Name
BCIP/NBT Substrate
Full Product Name
BCIP/NBT Substrate
Preparation and Storage
Store at 2-25°C.
Product Usage
BCIP/NBT Blue One Component AP Membrane Substrate contains 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium. The substrate is a ready to use solution. Unreacted substrate should be colorless to light yellow in appearance. When this substrate system is reacted with alkaline phosphatase, an insoluble permanent dark blue reaction product is obtained.
Substrates
BioFX™ Chemiluminescent Ultra Sensitive AP Microwell/Membrane Substrate (450 nm) (APU4)
BioFX™ AP Yellow One Component Microwell Substrate (pNPP)
BioFX™ Stabilizing Pellets for PNPS (STAB)
BioFX™ BCIP/NBT Purple One Component AP Membrane Substrate (BCID)
BioFX™ AP Yellow One Component Microwell Substrate (pNPP)
BioFX™ Stabilizing Pellets for PNPS (STAB)
BioFX™ BCIP/NBT Purple One Component AP Membrane Substrate (BCID)
Recommendations for use
This product should be allowed to equilibrate to room temperature (25°C) prior to use.
1) Use enough substrate solution to completely cover the membrane surface.
2) Rinse the membrane in distilled water to stop the reaction.
3) The reaction should be monitored and read before background color becomes too intense resulting in diminished contrast between positive and background staining.
1) Use enough substrate solution to completely cover the membrane surface.
2) Rinse the membrane in distilled water to stop the reaction.
3) The reaction should be monitored and read before background color becomes too intense resulting in diminished contrast between positive and background staining.
Product Categories/Family for BCIP/NBT Substrate substrate
Biological Reagents; Substrates
Application Notes for BCIP/NBT Substrate substrate
Additional considerations: Dilution of the substrate is not recommended. It is recommended that the antibodies or conjugates be diluted to reduce the intensity of the reaction.
Other Notes
Small volumes of BCIP/NBT Substrate substrate vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.