BirA, E. coli (His-MBP)
Cat# HY-P75475-50ug
Size : 50ug
Brand : MedChemExpress
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BirA Protein, E. coli (His-MBP)
- Cat. No.: HY-P75475
- COA Instruction de manipulation
BirA protein is not mentioned in the provided paragraph. If you have another paragraph or topic you'd like summarized with BirA as the subject, please provide it, and I'll be happy to assist. BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli, with N-His, N-MBP labeled tag. The total length of BirA Protein, E. coli (His-MBP) is 321 a.a., with molecular weight of 64-68 kDa.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
Size | Prix | Stock | Quantité |
---|---|---|---|
50 μg | $470 | En stock | |
100 μg | Obtenir un devis |
* Veuillez sélectionner la quantité avant d'ajouter des articles.
Activité biologique
Paramètres Techniques
Propriétés
Description
Description |
BirA protein is not mentioned in the provided paragraph. If you have another paragraph or topic you'd like summarized with BirA as the subject, please provide it, and I'll be happy to assist. BirA Protein, E. coli (His-MBP) is the recombinant E. coli-derived BirA protein, expressed by E. coli, with N-His, N-MBP labeled tag. The total length of BirA Protein, E. coli (His-MBP) is 321 a.a., with molecular weight of 64-68 kDa. |
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Background |
WBP2 protein functions as a transcriptional coactivator for estrogen and progesterone receptors (ESR1 and PGR) upon hormone activation, binding to ESR1-responsive promoters in the presence of estrogen. It is essential for the coactivation function of YAP1 on PGR activity and synergizes with WBP2 in enhancing PGR activity. Additionally, WBP2 modulates the expression of post-synaptic scaffolding proteins through the regulation of ESR1, ESR2, and PGR. WBP2 interacts with the WW domain of YAP1, WWP1, and WWP2, and also forms interactions with NEDD4. Moreover, it associates with ESR1 and UBE3A, contributing to its role as a transcriptional coactivator in hormone-responsive pathways. |
Activité biologique |
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet. |
Species |
E.coli |
Source |
E. coli |
Tag |
N-His;N-MBP |
Accession |
P06709 (M1-K321) |
Gene ID |
948469 [NCBI] |
Molecular Construction |
N-term
His-MBP
BirA (M1-K321)
Accession # P06709 C-term
|
Synonyms |
Bifunctional ligase/repressor BirA; birA; bioR; dhbB
|
Masse moléculaire |
64-68 kDa |
Pureté |
Greater than 95% as determined by reducing SDS-PAGE |
Appearance |
Lyophilized powder. |
Formulation |
Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. |
Endotoxin Level |
<1 EU/μg, determined by LAL method. |
Reconstitution |
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. |
Storage & Stability |
Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. |
Livraison |
Room temperature in continental US; may vary elsewhere. |
Documentation |
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BirA Protein, E. coli (His-MBP) Related Classifications
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Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.
Reconstitution Calculator
Dilution Calculator
Specific Activity Calculator
The reconstitution calculator equation
Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
The dilution calculator equation
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2
The specific activity calculator equation
Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Bulk Inquiry
Inquiry Information
- Nom du produit:
- BirA Protein, E. coli (His-MBP)
- Cat. No.:
- HY-P75475
- Quantité:
- MCE Japan Authorized Agent: