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> BMP-2 (Bone Morphogenetic Protein 2) BioAssay™ ELISA Kit (Equine)

BMP-2 (Bone Morphogenetic Protein 2) BioAssay™ ELISA Kit (Equine)

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Cat# 396572-96T

Size : 96Tests

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

396572BMP-2 (Bone Morphogenetic Protein 2) BioAssay™ ELISA Kit (Equine)

Specifications
Swiss ProtC8C060
ApplicationsE
Shipping TempBlue Ice
Storage Temp4°C/-20°C

Bone morphogenetic protein-2(BMP-2, BMP 2A) belongs to the transforming growth factor beta(TGF- beta) superfamily. It is thought to be involved in cartilage and bone formation during embryogenesis, but may have additional functions in morphogenesis as implied by its expression in various organs and embryonic tissues of mice.1 BMP-2 has been identified as a candidate mediator of retinoid activity. BMP-2 protein induces medulloblastoma cell apoptosis, whereas the BMP-2 antagonist noggin blocks both retinoid and BMP-2-induced apoptosis. BMP-2 also induces p38 mitogen-activated protein kinase(MAPK), which is necessary for BMP-2 and retinoid-induced apoptosis.2 Bone morphogenic proteins(BMPs) are known to promote osteogenesis, and clinical trials are currently underway to evaluate the ability of certain BMPs to promote fracture-healing and spinal fusion.3 The standard product used in this kit is recombinant equine BMP-2, constituting dimer by two chains of 114aa with the molecular mass of 26kD.

Sample Type:
Cell culture supernates, serum, bone tissue, tissue.

Intended Use:
Sandwich High Sensitivity BioAssay™ ELISA kit for Quantitative Detection of Equine BMP-2.

Sensitivity:
<2pg/ml

Range:
31.2pg/ml-2000pg/ml

Specificity:
Recognizes natural and recombinant Equine BMP-2. There is no detectable cross-reactivity with other relevant proteins.

Test Principle:
This Equine BMP-2 BioAssay™ ELISA Kit was based on standard sandwich enzyme-linked immune- sorbent assay technology. A monoclonal antibody from mouse specific for Equine BMP-2 has been precoated onto 96-well plates. Standards(Expression system for standard: CHO; Immunogen sequence: Q283-R396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Equine BMP-2 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Equine BMP-2 amount of sample captured in plate.

Kit Components:
96-well plate precoated with anti-Equine BMP-2 antibody, 1x plate
Lyophilized recombinant Equine BMP-2 standard, 2x10ng/tube
Biotinylated anti-Equine BMP-2 antibody, 1x130ul (dilution 1:100)
Avidin-Biotin-Peroxidase Complex (ABC), 1x130ul (dilution 1:100)
Sample diluent buffer, 1x30ml
Antibody diluent buffer, 1x12ml
ABC diluent buffer, 1x12ml
TMB color developing agent, 1x10ml
TMB stop solution, 1x10ml
PBS washing buffer Powder for 1000ml, 1x buffer

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Applications
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. Toxicity and Hazards: All products should be handled by qualified personnel only, trained in laboratory procedures.
References
1. Gopal Rao, V. V. N.; Loffler, C.; Wozney, J. M.; Hansmann, I. The gene for bone morphogenetic protein 2A (BMP2A) is localized to human chromosome 20p12 by radioactive and nonradioactive in situ hybridization. Hum. Genet. 90: 299-302, 1992.|2. Hallahan, A. R.; Pritchard, J. I.; Chandraratna, R. A. S.; Ellenbogen, R. G.; Geyer, J. R.; Overland, R. P.; Strand, A. D.; Tapscott, S. J.; Olson, J. M. BMP-2 mediates retinoid-induced apoptosis in medulloblastoma cells through a paracrine effect. Nature Med. 9: 1033-1038, 2003.|3. Cheng, H.; Jiang, W.; Phillips, F. M.; Haydon, R. C.; Peng, Y.; Zhou, L.; Luu, H. H.; An, N.; Breyer, B.; Vanichakarn, P.; Szatkowski, J. P.; Park, J. Y.; He, T.-C. Osteogenic activity of the fourteen types of human bone morphogenetic proteins (BMPs). J. Bone Joint Surg. 85-A: 1544-1552, 2003.