BseSI
Cat# MBS638425-500U
Size : 500Units
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
Bsesi Restriction Enzyme
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS638425MBS638425 is a restriction enzyme targeting BseSI. G A More details
Product Overview
Online Datasheet in PDFProduct Name
BseSI, Restriction Enzyme
Full Product Name
BseSI
Purity/Purification
Molecular Biology Grade
Preparation and Storage
-20 degree C
Dilution Buffer
10mM Tris-HCl (pH 7.4 at 25 degree C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer.
Digestion of Agarose-embedded DNA
A minimum of 5units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours.
Compatible Ends
GGGCC^C-ApaI, Eco24I, SduI GTGCA^C-Alw21I, Mph1103I, PstI, SdaI, SduI
pACYC177
2
pACYC184
1
Labeled Oligonucleotide (LO) Assay
No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10units of restriction endonuclease for 4 hours.
Lambda
10
Ligation/Recutting Assay
After 50-fold overdigestion (3u/ug DNA x 17 hours) with BseSI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut.
M13mp18/19
2*
Overdigestion Assay
No detectable change in the specific fragmentation pattern is observed after 80-fold overdigestion (5u/ug lambda DNA x 16 hours) with BseSI (see Star Activity).
pBR322
3
PhiX174
1
pTZ19R/U
2
pUC18/19
3
pUC57
4
Stability During Prolonged Incubation
A minimum of 0.1units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 55 degree C.
Storage Buffer
10mM Tris-HCl (pH 7.5 at 25 degree C), 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol.
Thermal Inactivation
Only small amounts of enzyme (up to 10units) can be inactivated 80 degree C for 20min.
Unit Definition
One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 55 degree C in 50ul of assay buffer.
Product Categories/Family for BseSI restriction enzyme
Molecular Biology; MB-Enzymes; Restriction
Other Notes
Small volumes of BseSI restriction enzyme vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.