CARD4/NOD1 Antibody
Cat# orb669193-100ug
Size : 100ug
Brand : Biorbyt
Contact local distributor :
Phone : +1 850 650 7790
CARD4/NOD1 Antibody
Catalog Number: orb669193
| Catalog Number | orb669193 |
|---|---|
| Category | Antibodies |
| Description | CARD4/NOD1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | WB |
| Reactivity | Monkey |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence of human CARD4/NOD1 (NQTLKHLWLIQNQITAK). |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Dilution range | Western blot, 0.25-0.5μg/ml, Monkey |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| MW | 108 kDa |
| UniProt ID | Q9Y239 |
| Storage | Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
| Note | For research use only |
| Application notes | Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. Add 0.2ml of distilled water will yield a concentration of 500ug/ml. |
| Expiration Date | 12 months from date of receipt. |
Western blot analysis of CARD4/NOD1 using anti-CARD4/NOD1 antibody (orb669193). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: monkey heart tissue lysates, Lane 2: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD4/NOD1 antigen affinity purified polyclonal antibody (Catalog # orb669193) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for CARD4/NOD1 at approximately 108KD. The expected band size for CARD4/NOD1 is at 108KD.