| Size | |
|---|---|
| 100µg | Inquiry |
Dispatch Time: About 3 working days
- Product Name
- CD274 antibody
- Catalogue No.
- FNab06281
- Size
- 100μg
- Form
- liquid
- Purification
- Protein A+G purification
- Purity
- ≥95% as determined by SDS-PAGE
- Clonality
- monoclonal
- Isotype
- IgG1
- Clone ID
- 6C11
- Storage
- PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20℃ for 12 months(Avoid repeated freeze / thaw cycles.)
Immunogen
- Immunogen
- CD274 molecule
- Alternative Names
- Programmed cell death 1 ligand 1 (PD-L1 antibody, PDCD1 ligand 1 antibody, Programmed death ligand 1 antibody, hPD-L1)|B7 homolog 1 (B7-H1)|CD274|B7H1|PDCD1L1|PDCD1LG1|PDL1 antibody
- UniProt ID
- Q9NZQ7
- Observed MW
- 40-50 kDa
Application
- Tested Applications
- ELISA, WB, IHC,IF
- Recommended dilution
- WB: 1:500-1:5000; IHC: 1:100-1:500; IF: 1:50-1:500
Validated Images
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with FNab06281(PD-L1/CD274 Antibody) at dilution of 1:1000 
Immunohistochemistry of paraffin-embedded human tonsillitis tissue slide using FNab06281(PD-L1/CD274 Antibody) at dilution of 1:50 . heat mediated antigen retrieved with Tris-EDTA buffer(pH9). 
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using FNab06281 (PD-L1/CD274 Antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) - Background
- Programmed cell death ligand 1(CD274, or B7-H1, PD-L1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. CD274 is suggested a negative regulator of T and B cell, and play important role in mediating tolerance of lymphocytes to self-antigens. It also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, the apparent molecular weight has been reported as 45-70 kDa, suggesting probable glycosylation(PMID: 18760278; 16493058).?