CD4 (Biotin)

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Cat# 227417-Biotin-100ul

Size : 100ul

Contact local distributor :

Phone : +1 850 650 7790

Brand : US Biological

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Contact local distributor :

Phone : +1 850 650 7790

227417-Biotin CD4 (Biotin)

Clone Type

Monoclonal

Host

mouse

Source

equine

Swiss Prot

F6Y6X8

Isotype

IgG1

Grade

Affinity Purified

Applications

FC IHC IP

Crossreactivity

Shipping Temp

Blue Ice

Storage Temp

-20°C

CD4 is a 58kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes. As in humans, equine CD4 expression is mutually exclusive with CD8 expression on mature T-cells.

A study undertaken using 227417, to identify CD4 on several wild african equid species, indicates that CD4 recognizes Somali wild ass (Equus asinus) but not Grévy's Zebra (E. grevyi) or Hartmann's Mountain Zebra (E. zebra).

Applications:
Suitable for use in Flow Cytometry, Immunoprecipitation, and Immunohistochemistry. Other applications have not been tested.d.

Recommended Dilution:
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Hybridoma:
X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications

Product Type: Mab|Isotype: IgG1|Clone No: CVS4|Host: mouse|Source: equine|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.|Purity: Purified by Protein A affinity chromatography form tissue culture supernatant.|Immunogen: Equine Thymocytes|Specificity: Recognizes equine CD4.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

Immunogen

Equine Thymocytes

Form

Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

Purity

Purified by Protein A affinity chromatography form tissue culture supernatant.

Specificity

Recognizes equine CD4.

References

1. Lunn, D.P.et al. (1991) Three monoclonal antibodies identifying antigens on all equine T lymphocytes, and two mutually exclusive T-lymphocyte subsets. Immunol. 74: 251-257. 2. Kydd, J.H. and Antczak, D.F., (1991) Report of the First International Workshop on Equine Leucocyte Antigens, Cambridge, UK, July 1991 Equine Immunol. 4-5. 3. Deeg,C.A. et al. (2004) The uveitogenic potential of retinal S-antigen in horses. Invest Ophthalmol Vis Sci. 45: 2286-92. 4. Pearson, W. et al. (2007) Low-dose ginseng (Panax quinquefolium) modulates the course and magnitude of the antibody response to vaccination against equid herpesvirus I in horses. Can J Vet Res. 71: 213-7. 5. Brault, S.A. et al. (2010) The immune response of foals to natural infection with equid herpesvirus-2 and its association with febrile illness. Vet Immunol Immunopathol. 137: 136-41. 6. Goodman, L.B. et al. (2007) A point mutation in a herpesvirus polymerase determines neuropathogenicity. PLoS Pathog. 3(11):e160. 7. Hamza, E. et al. (2011) CD4(+)CD25(+) T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol. Jun 6. [Epub ahead of print]. 8. Go, Y.Y. et al. (2010) Complex interactions between the major and minor envelope proteins of equine arteritis virus determine its tropism for equine CD3+ T lymphocytes and CD14+ monocytes. J Virol. 84: 4898-911. 9. Lunn, D.P. et al. (1998) Report of the Second Equine Leucocyte Antigen Workshop, Squaw valley, California, July 1995. Vet Immunol Immunopathol. 62: 101-143. 10. Ibrahim, S. (2007) Analysis of monoclonal antibody cross-reactivity with leukocytes from equids and cloning of CD28. Anchor Text.