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> CD47 (APC)

CD47 (APC)

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Cat# C2402-04-APC-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2402-04-APCCD47 (APC)

Specifications
Clone TypeMonoclonal
Hostmouse
Sourcerat
Swiss ProtP97829
IsotypeIgG1
GradeAffinity Purified
ApplicationsIP WB
CrossreactivityRt
Shipping TempBlue Ice
Storage Temp4°C Do Not Freeze

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilution determined by the researcher.

Hybridoma:
Spleen cells from immunized Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.

Recommended Secondary Reagents:
IgG Goat anti mouse IgG:FITC (rat adsorbed)
IgG Goat anti mouse IgG:RPE (rat adsorbed)

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG1|Clone No: 3H1457 (OX-101)|Host: mouse|Source: rat|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: Rat thymocytes.|Specificity: Recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
Rat thymocytes.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes the rat homologue of human CD47, which has been identified as the ligand for the rat signal regulatory protein (SIRP). Rat CD47 is expressed primarily by lymphocytes and macrophages but has also been detected at high levels on brain endothelial cells.
References
1. Vernon-Wilson, E. F. et al. (2000). CD47 is a ligand for rat macrophage membrane signal regulatory protein SIRP (OX41) and human SIRP alph a 1. Eur. J. Immunol. 30: 2130-2137. 2. de Vries, H.E. et al. (2002). Signal regulatory protein alpha - CD47 interactions are required for the transmigration of monocytes across cerebral endothelium. J. Immunol. 168: 5832 - 5839.