Reagents and instruments for immunology, cell biology and molecular biology.
 
> CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (HRP)

CD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (HRP)

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Cat# C2405-04V-HRP-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

C2405-04V-HRPCD49f (CD49f Antigen, Integrin alpha 6, ITGA6, ITGA6B, VLA 6 alpha Subunit, VLA-6) (HRP)

Specifications
Clone TypeMonoclonal
Hostrat
Sourcehuman
IsotypeIgG2a
GradeAffinity Purified
ApplicationsIHC IP
CrossreactivityCa Hu Mk Mo Po Sh
Shipping TempBlue Ice
Storage Temp-20°C

CD49f can form distinct complexes with either the CD29-antigen (VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells. Platelet VLA-6 functions as a laminin receptor.

Applications:
Suitable for use in Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen): Reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts.
Optimal dilutions to be determined by the researcher.

IHC Postive Control:
Human Tonsil

Hybridoma:
Sp2/0 myeloma cells with spleen cells from immunized Sprague-Dawley rats.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a|Clone No: 3H1515 (NKI-GoH3)|Host: rat|Source: human|Concentration: As Reported|Form: Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).|Purity: Purified by Protein G affinity chromatography from tissue culture supernatant.|Immunogen: BALB/c mouse mammary tumor cells.|Specificity: Recognizes human CD49f. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). Reported to inhibit platelet adhesion to laminin. Species Crossreactivity: Mouse, canine, porcine, sheep, cynomolgus monkey.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
BALB/c mouse mammary tumor cells.
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with horseradish peroxidase (HRP).
Purity
Purified by Protein G affinity chromatography from tissue culture supernatant.
Specificity
Recognizes human CD49f. Reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). Reported to inhibit platelet adhesion to laminin. Species Crossreactivity: Mouse, canine, porcine, sheep, cynomolgus monkey.
References
1. Soligo, D. et al. (1989) Immunohistochemical reacitivy on bone marrow and tissues of anti-VLA antibodies in the platelet panel, in Leucocyte Typing IV: White Cell Differentiation Antigens. Edited by Knapp, W. et al. Oxford University Press p1029-1032, 2. Workshop of the 4th International Conference on Human Leucocyte Differentiation Antigens Vienna (1989) Workshop number p 055. Oxford University Press, 3. Sonnenberg, A. et al. (1986) Development of mouse mammary gland: identification of stages in differentiation of luminal and myoepithelial cells using monoclonal antibodies and polyvalent antiserum against keratin. Histochem. Cytochem. 34: 1037-1046, 4. Sonnenberg, A. et al . (1987) A complex of platelet glycoproteins Ic and IIa identified by a rat monoclonal antibody J. Biol. Chem. 262: 10376-10383, 5. Helmer, M. et al. (1988) Multiple very late antigen (VLA) heterodimers on platelets. Evidence for distinct VLA-2, VLA-5 (fibronectin receptor), and VLA-6 structures J. Biol. Chem. 263: 7660-7665, 6. Galkowska, H. et al. (1996) Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes. Vet. Immunol. Immunopathol. 53: 329 - 334, 7. Sonnenberg, A. et al. (1988) Laminin receptor on platelets is the integrin VLA-6. Nature. 336: 487 - 489, 8. Sonnenberg, A. et al. (1990) Integrin recognition of different cell-binding fragments of laminin (P1, E3, E8) and evidence that alpha 6 beta 1 but not alpha 6 beta 4 functions as a major receptor for fragments E8. J. Cell. Biol. 110: 2145 - 2155, 9. Sonnenberg, A., et al. (1988) Identification and characterization of a novel antigen complex on mouse mammary tumour cells using a monoclonal antibody against platelet glycoprotein Ic. J. Biol. Chem. 263: 14030 - 14038, 10. Yoshino, N. et al. (2000) Upgrading of flow cytometric analysis for absolute counts, cytokines and other antigenic molecules of Cynomolgus monkeys (Macaca fascicularis) by using anti-human cross-reactive antibodies. Exp. Anim.49 (2): 97-110, 11. Sonneberg, A. et al. (1990) The alpha 6 beta 1 (VLA-6) and alpha 6 beta 4 protein complexes: tissue distribution and biochemical properties. J. Cell Sci. 96: 207 - 217.