Clenbuterol ELISA Kit

Cat# OKAO00117

Size : 96Wells

Brand : Aviva Systems Biology

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Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for Clenbuterol ELISA Kit (OKAO00117)
Product Info
Predicted Species ReactivityGeneral
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~4 Hours
ELISA Kit PrincipleAviva Systems Biology Clenbuterol ELISA Kit (OKAO00117) is based on standard competitive inhibition enzyme-linked immuno-sorbent assay technology. Clenbuterol has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells along with a rabbit anti-Clenbuterol antibody and an anti-rabbit / HRP Conjugated antibody, then incubated. Wells are washed and detection substrates are added and incubated. An enzymatic reaction is produced through the addition of TMB which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm which is quantitatively proportional to the amount of detector antibody bound in the well and inversely proportional to the amount of Sulfadimethoxine in the sample.
ELISA Kit Range0.05-4.05 ppb
ELISA Kit Recovery
SampleAmount
Urine95% +/- 10%
Tissue85% +/- 15%
Feed85% +/- 15%
ELISA Kit ReproducibilityMean Intra-Assay: CV<8% (n= 3 x 20)
Mean Inter-Assay: CV<12% (n= 3 x 40)
ELISA Kit Component
ComponentAmount
Clenbuterol Microplate96 Wells (12 x 8 Well Strips)
Clenbuterol Standards6 x 1 mL
Clenbuterol Rabbit Detector Antibody1 x 6 mL
Anti-Rabbit / HRP Conjugate1 x 6 mL
Detection Reagent A1 x 6 mL
Detection Reagent B1 x 6 mL
Stop Solution1 x 6 mL
20X Wash Buffer1 x 20 mL
10X Sample Prep Buffer1 x 20 mL
Additional InformationCAS: 93106-60-6
PubChem: 2783
::Molecular Formula: C12H18Cl2N2O
Reconstitution and StorageStore as indicated in product manual.
Sample TypeUrine, tissue, feed
Sensitivity
SampleAmount
Urine0.05 ppb
Tissue (1)0.2 ppb
Tissue (2)0.05 ppb
Feed0.5 ppb
Specificity
SubstanceCross Reactivity Rate
Clenbuterol100%
Terbutalin1%
Mabuterol1%
Brombuterol1%
Salbutamal1%
Ractopamine1%
Assay InfoAssay Methodology: Competitive Inhibition ELISA
Alias SymbolsCLE, Clenbuterol, Clenbuterolum, 37148-27-9, Monores, NAB-365, Clenbuterolum
Protein NameClenbuterol
Description of TargetClenbuterol is a substituted phenylaminoethanol that has beta-2 adrenomimetic properties at very low doses. It is used as a bronchodilator in asthma.
Molecular Weight277.19012 g/mol