CNP siRNA (Mouse)
Cat# MBS827235-15nmol
Size : 15nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
CNP siRNA – 2',3'-Cyclic-Nucleotide 3'-Phosphodiesterase Isoform 1 (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827235MBS827235 is an siRNA targeting CNP. Also known as CNP siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit CNP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
CNP, siRNA
Full Product Name
CNP siRNA (Mouse)
Product Gene Name
CNP sirna
Product Synonym Names
CNP1; 2'.3'-cyclic-nucleotide 3'-phosphodiesterase; CNP; CNPase
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
CNP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
44,654 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse CNP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for CNP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of CNP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for CNP. It may not necessarily be applicable to this product.
NCBI Official Full Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase isoform 1
NCBI Official Synonym Full Names
2',3'-cyclic nucleotide 3' phosphodiesterase
NCBI Official Symbol
Cnp
NCBI Official Synonym Symbols
Cnp1; Cnp-1; CNPase
UniProt Protein Name
2',3'-cyclic-nucleotide 3'-phosphodiesterase
Protein Family
2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProt Gene Name
Cnp
UniProt Synonym Gene Names
Cnp1; CNP; CNPase
UniProt Entry Name
CN37_MOUSE
NCBI Protein Information
2',3'-cyclic-nucleotide 3'-phosphodiesterase
UniProt Comments for CNP
CNP: May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin. Exists as monomers and homodimers. Belongs to the cyclic nucleotide phosphodiesterase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; EC 3.1.4.37; Phosphodiesterase
Cellular Component: extracellular space; microtubule; microvillus; pseudopodium; nucleoplasm; mitochondrial outer membrane; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; nucleus; myelin sheath
Molecular Function: cyclic nucleotide binding; hydrolase activity; RNA binding; 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
Biological Process: axonogenesis; adult locomotory behavior; response to toxin; oligodendrocyte differentiation; microtubule cytoskeleton organization and biogenesis; regulation of mitochondrial membrane permeability; cyclic nucleotide catabolic process
Show more Protein type: Cell development/differentiation; EC 3.1.4.37; Phosphodiesterase
Cellular Component: extracellular space; microtubule; microvillus; pseudopodium; nucleoplasm; mitochondrial outer membrane; cell projection; membrane; perinuclear region of cytoplasm; cytoplasm; mitochondrial inner membrane; plasma membrane; nucleus; myelin sheath
Molecular Function: cyclic nucleotide binding; hydrolase activity; RNA binding; 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity
Biological Process: axonogenesis; adult locomotory behavior; response to toxin; oligodendrocyte differentiation; microtubule cytoskeleton organization and biogenesis; regulation of mitochondrial membrane permeability; cyclic nucleotide catabolic process
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with CNP sirna
| Reference Product | PubMed Publications |
|---|---|
| Olig2 sirna | >23 publications with CNP and Olig2 |
| Plp1 sirna | >16 publications with CNP and Plp1 |
| Sox10 sirna | >12 publications with CNP and Sox10 |
| Galc sirna | >9 publications with CNP and Galc |
| Olig1 sirna | >5 publications with CNP and Olig1 |
| Aif1 sirna | >2 publications with CNP and Aif1 |
| Tubb3 sirna | >2 publications with CNP and Tubb3 |
| Nefh sirna | >1 publications with CNP and Nefh |
Diseases associated with CNP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >259 publications with CNP and Nervous System Diseases |
| Brain Diseases Antibodies | >193 publications with CNP and Brain Diseases |
| Hypertension Antibodies | >120 publications with CNP and Hypertension |
| Disease Models, Animal Antibodies | >101 publications with CNP and Disease Models, Animal |
| Inflammation Antibodies | >75 publications with CNP and Inflammation |
| Immune System Diseases Antibodies | >66 publications with CNP and Immune System Diseases |
| Kidney Diseases Antibodies | >56 publications with CNP and Kidney Diseases |
| Mental Disorders Antibodies | >48 publications with CNP and Mental Disorders |
| Hypertrophy Antibodies | >43 publications with CNP and Hypertrophy |
| Fibrosis Antibodies | >43 publications with CNP and Fibrosis |
Organs/Tissues associated with CNP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >911 publications with CNP and Brain |
| Blood Antibodies | >545 publications with CNP and Blood |
| Heart Antibodies | >335 publications with CNP and Heart |
| Kidney Antibodies | >188 publications with CNP and Kidney |
| Bone Antibodies | >127 publications with CNP and Bone |
| Spinal Cord Antibodies | >116 publications with CNP and Spinal Cord |
| Lung Antibodies | >96 publications with CNP and Lung |
| Liver Antibodies | >81 publications with CNP and Liver |
| Embryonic Tissue Antibodies | >69 publications with CNP and Embryonic Tissue |
| Eye Antibodies | >53 publications with CNP and Eye |