Complement 4 (C4) BioAssay™ ELISA Kit (Canine)

For the quantitative determination of canine Complement 4 (C4) in serum, plasma, and tissue homogenates.

Sensitivity:
7.81ng/ml

Detection Range:
31.25-2000ng/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of canine C4. No significant cross-reactivity or interference between canine C4 and analogues was observed.

Assay Principle:
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with C4. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for C4. The competitive inhibition reaction is launched between with pre-coated C4 and C4 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of C4 in the samples. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard (Freeze dried), 2xvial
Biotin-antibody (100X), 1x120ul
HRP-avidin (100X), 1x120ul
Biotin-antibody Diluent, 1x15ml
HRP-avidin Diluent, 1x15ml
Sample Diluent, 1x50ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution: 1x10ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.