Complement Fragment 5a, C5a BioAssay™ ELISA Kit (Rabbit)

For the quantitative determination of rabbit complement fragment 5a in serum, plasma, and tissue homogenates.

Sensitivity:
0.156ng/ml

Detection Range:
0.625-40ng/ml

Specificity:
This assay has high sensitivity and excellent specificity for detection of rabbit C5a. No significant cross-reactivity or interference between rabbit C5a and analogues was observed.

Assay Principle:
This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for C5a has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any C5a present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for C5a is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of C5a bound in the initial step. The color development is stopped and the intensity of the color is measured.

Kit Components:
Microtiter Strips, 1x96 wells
Standard (Freeze dried), 2xvial
Biotin-antibody (100X), 1x120ul
HRP-avidin (100X), 1x120ul
Biotin-antibody Diluent, 1x15ml
HRP-avidin Diluent, 1x15ml
Sample Diluent, 1x50ml
Wash Buffer (25X), 1x20ml
TMB Substrate, 1x10ml
Stop Solution: 1x10ml

Storage and Stability:
Store all kit components at 4°C. Stable for 6 months after receipt. Once opened, the kit is stable for 1 month providing this is within the expiration date of the kit. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.