CXCL2 ELISA Kit (Rat)

Cat# OKEH02971

Size : 96Wells

Brand : Aviva Systems Biology

Contact local distributor :


Phone : +1 850 650 7790

Datasheets/ManualsPrintable datasheet for CXCL2 ELISA Kit (Rat) (OKEH02971)
Product Info
Predicted Species ReactivityRat|Rattus norvegicus
ApplicationELISA-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology CXCL2 ELISA Kit (Rat) (OKEH02971) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cxcl2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Cxcl2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Cxcl2 captured in well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Cxcl2 Microplate96 Wells (12 x 8 Well strips)
Cxcl2 Lyophilized Standard2
100X Biotinylated Cxcl2 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity7.91 pg/mL
SpecificityNatural and recombinant Rat C-X-C motif chemokine 2
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolCxcl2
Gene Full NameC-X-C motif chemokine ligand 2
Alias SymbolsC-X-C motif chemokine 2, chemokine (C-X-C motif) ligand 2, CINC-3, cytokine-induced neutrophil chemoattractant 3, macrophage inflammatory protein 2, Mip-2, MIP2, Scyb2, small inducible cytokine subfamily, member 2.
NCBI Gene Id114105
Protein NameC-X-C motif chemokine 2
Description of TargetChemotactic for human polymorphonuclear leukocytes but does not induce chemokinesis or an oxidative burst. Contributes to neutrophil activation during inflammation.
Uniprot IDP30348
Protein Accession #NP_446099.1
Nucleotide Accession #NM_053647.1
Protein Size (# AA)100
Molecular Weight11 kDa

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