Cycloheximide is an antibiotic produced by S. griseus. Its main biological activity is translation inhibition in eukaryotes, resulting in inhibition of protein synthesis leading to cell growth, cell arrest and cell death. CHX is widely used for selection of CHX resistant yeast and fungi strains; controlled inhibition of protein synthesis for detection of short-lived proteins and super-induction of protein expression; apoptosis induction or facilitation of apoptosis induction of death receptors.

Prepared from C8500 Cycloheximide

Synonyms:
4-[(2R)-2-[(1S,3S,5S)-3,5-Dimethyl-2-oxocyclohexyl]-2-hydroxyethyl]-2,6-piperidinedione; 3-[2-(3,5-Dimethyl-2-oxocyclohexyl)-2-hydroxyethyl]glutarimide; Naramycin A; Acti-Aid; Acti-dione; Acti-dione BR; Acti-dione PM; Acti-dione TGF; Actidion; CHX; Cicloheximide; FT 3422-2; NSC 185; Naramycin; Naramycin Microcapsule D 80; Naramycin NM-MCU 80; TZA; U 4527; β-[2-(3,5-Dimethyl-2-oxocyclohexyl)-
2-hydroxyethyl]glutarimide

CAS No:
66-81-9

Molecular Formula:
C15H23NO4

Molecular Weight:
281.35

Source:
S. griseus

Purity:
≥95%

Appearance:
Colorless to light yellow, clear, complete. Supplied as a sterile-filtered (0.2um) liquid in 95% ethanol.

Concentration:
50mg/ml

Specific Rotation (C=1, CHCl3):
-25° to -32°

Storage and Stability:
For long term storage, aliquot and store at -20°C. Stable for 12 months at -20°C after receipt.