DNMT1 siRNA (Mouse)
Cat# MBS8214961-5x30nmol
Size : 5x30nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
DNMT1 siRNA – DNA (Cytosine-5)-Methyltransferase 1 Isoform 1 (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS8214961MBS8214961 is an siRNA targeting DNMT1. Also known as DNMT1 siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit DNMT1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
DNMT1, siRNA
Full Product Name
DNMT1 siRNA (Mouse)
Product Gene Name
DNMT1 sirna
Product Synonym Names
DNMT; MET1; UIM; DNA; cytosine-5)-methyltransferase 1; Dnmt1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; M.MmuI; MCMT
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
DNMT1 siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
169,996 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse DNMT1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for DNMT1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of DNMT1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for DNMT1. It may not necessarily be applicable to this product.
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform 1
NCBI Official Synonym Full Names
DNA methyltransferase (cytosine-5) 1
NCBI Official Symbol
Dnmt1
NCBI Official Synonym Symbols
Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Synonym Protein Names
DNA methyltransferase MmuI; DNA MTase MmuI; M.MmuI; MCMT
Protein Family
DNA
UniProt Gene Name
Dnmt1
UniProt Synonym Gene Names
Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
UniProt Entry Name
DNMT1_MOUSE
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1
UniProt Comments for DNMT1
DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.
Protein type: EC 2.1.1.37; Transcription regulation; Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase
Cellular Component: centric heterochromatin; heterochromatin; protein complex; cell soma; cytoplasm; replication fork; nucleus
Molecular Function: protein domain specific binding; methyltransferase activity; methyl-CpG binding; unmethylated CpG binding; zinc ion binding; RNA binding; metal ion binding; histone deacetylase binding; transcription factor binding; DNA-methyltransferase activity; transferase activity; DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; double-stranded DNA binding; estrogen receptor binding; chromatin binding; catalytic activity
Biological Process: negative regulation of histone H3-K9 methylation; methylation; maintenance of DNA methylation; transcription, DNA-dependent; metabolic process; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; gene silencing; chromatin modification; regulation of cell proliferation; cytosine methylation within a CG sequence; regulation of transcription, DNA-dependent; regulation of gene expression; DNA methylation; S-adenosylmethioninamine metabolic process; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine
Show more Protein type: EC 2.1.1.37; Transcription regulation; Methyltransferase, DNA; Cell development/differentiation; Amino Acid Metabolism - cysteine and methionine; Methyltransferase
Cellular Component: centric heterochromatin; heterochromatin; protein complex; cell soma; cytoplasm; replication fork; nucleus
Molecular Function: protein domain specific binding; methyltransferase activity; methyl-CpG binding; unmethylated CpG binding; zinc ion binding; RNA binding; metal ion binding; histone deacetylase binding; transcription factor binding; DNA-methyltransferase activity; transferase activity; DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; double-stranded DNA binding; estrogen receptor binding; chromatin binding; catalytic activity
Biological Process: negative regulation of histone H3-K9 methylation; methylation; maintenance of DNA methylation; transcription, DNA-dependent; metabolic process; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; gene silencing; chromatin modification; regulation of cell proliferation; cytosine methylation within a CG sequence; regulation of transcription, DNA-dependent; regulation of gene expression; DNA methylation; S-adenosylmethioninamine metabolic process; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with DNMT1 sirna
| Reference Product | PubMed Publications |
|---|---|
| Dnmt3a sirna | >566 publications with DNMT1 and Dnmt3a |
| Dnmt3b sirna | >552 publications with DNMT1 and Dnmt3b |
| Hdac1 sirna | >116 publications with DNMT1 and Hdac1 |
| Uhrf1 sirna | >76 publications with DNMT1 and Uhrf1 |
| Pcna sirna | >66 publications with DNMT1 and Pcna |
| Hdac2 sirna | >38 publications with DNMT1 and Hdac2 |
| Ezh2 sirna | >38 publications with DNMT1 and Ezh2 |
| Suz12 sirna | >5 publications with DNMT1 and Suz12 |
| Smarca5 sirna | >5 publications with DNMT1 and Smarca5 |
| Baz2a sirna | >1 publications with DNMT1 and Baz2a |
Pathways associated with DNMT1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| Cysteine And Methionine Metabolism Pathway antibodies | Cysteine And Methionine Metabolism Pathway Diagram |
| DNA Methylation Pathway antibodies | DNA Methylation Pathway Diagram |
| Epigenetic Regulation Of Gene Expression Pathway antibodies | Epigenetic Regulation Of Gene Expression Pathway Diagram |
| Gene Expression Pathway antibodies | Gene Expression Pathway Diagram |
| Glutathione And One Carbon Metabolism Pathway antibodies | Glutathione And One Carbon Metabolism Pathway Diagram |
| Metabolic Pathways antibodies | Metabolic Pathways Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| Methionine Degradation Pathway antibodies | Methionine Degradation Pathway Diagram |
| MicroRNAs In Cancer Pathway antibodies | MicroRNAs In Cancer Pathway Diagram |
Diseases associated with DNMT1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >242 publications with DNMT1 and Carcinoma |
| Adenocarcinoma Antibodies | >109 publications with DNMT1 and Adenocarcinoma |
| Breast Neoplasms Antibodies | >94 publications with DNMT1 and Breast Neoplasms |
| Liver Diseases Antibodies | >67 publications with DNMT1 and Liver Diseases |
| Cell Transformation, Neoplastic Antibodies | >66 publications with DNMT1 and Cell Transformation, Neoplastic |
| Disease Models, Animal Antibodies | >65 publications with DNMT1 and Disease Models, Animal |
| Carcinoma, Hepatocellular Antibodies | >63 publications with DNMT1 and Carcinoma, Hepatocellular |
| Lung Neoplasms Antibodies | >60 publications with DNMT1 and Lung Neoplasms |
| Liver Neoplasms Antibodies | >56 publications with DNMT1 and Liver Neoplasms |
| Neoplasms, Experimental Antibodies | >47 publications with DNMT1 and Neoplasms, Experimental |
Organs/Tissues associated with DNMT1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >148 publications with DNMT1 and Brain |
| Liver Antibodies | >129 publications with DNMT1 and Liver |
| Lung Antibodies | >105 publications with DNMT1 and Lung |
| Embryonic Tissue Antibodies | >51 publications with DNMT1 and Embryonic Tissue |
| Skin Antibodies | >38 publications with DNMT1 and Skin |
| Kidney Antibodies | >36 publications with DNMT1 and Kidney |
| Bone Antibodies | >35 publications with DNMT1 and Bone |
| Testis Antibodies | >31 publications with DNMT1 and Testis |
| Bone Marrow Antibodies | >27 publications with DNMT1 and Bone Marrow |
| Heart Antibodies | >23 publications with DNMT1 and Heart |