ELISA Wash Buffer, 10X
Cat# MBS258403-5x1L
Size : 5x1L
Brand : MyBiosource
Contact local distributor :
Phone : +1 850 650 7790
ELISA Wash Buffer
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258403Description: ELISA Wash Buffer, 10X effectively rinses microtiter plates between reagent addition steps of an ELISA to remove signal-altering debris yet preserve assay components.Background: ELISA Wash Buffer formulation is used to rinse microtiter plates… More details
Product Overview
Online Datasheet in PDFProduct Name
ELISA Wash Buffer
Full Product Name
ELISA Wash Buffer, 10X
Product Synonym Names
ELISA Wash Buffer; 10X
Form/Format
Supplied at 10X.
Preparation and Storage
Store at room temperature or 2-8 degree C.
pH
7.2 at 1X
Contains
No proteins.
Product Categories/Family for ELISA Wash Buffer buffer
ELISA Solutions; Wash Buffer
Application Notes for ELISA Wash Buffer buffer
1. Dilute ELISA Wash Buffer, 10X by adding 1 part ELISA Wash Buffer to 9 parts deionized water and mix for 15 minutes. ELISA Wash Buffer, 10X is supplied concentrated, so crystalline precipitates may form in the bottle, especially when refrigerated. If this happens, gently warm or mix the buffer until all crystals are dissolved. Do not let it boil.
2. Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 uL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system.
3. Aspirate or dump out the buffer and repeat for a total of 2-4 washes.
4. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid.
If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.
2. Completely fill the wells of the ELISA plate with 1X wash buffer (about 400 uL/well). The diluted wash buffer may be dispensed through a squirt bottle, a plate washer, through a multi-channel pipette, or through an automated system.
3. Aspirate or dump out the buffer and repeat for a total of 2-4 washes.
4. After the final dump or aspiration, pound the plate on paper towels to remove any excess liquid.
If washing the plate during the coating process, we recommend 2-3 washes after the coating step before adding the block buffer. If washing the plate as part of an ELISA procedure, we recommend 3-4 washes after the sample incubation and after the conjugate incubation.
Other Notes
Small volumes of ELISA Wash Buffer buffer vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for ELISA Wash Buffer purchase
MBS258403 is a buffer targeting ELISA Wash Buffer. Also known as ELISA Wash Buffer, 10X.