Reagents and instruments for immunology, cell biology and molecular biology.
 
> Eosin Y

Eosin Y

Print Print
Please log in

Cat# orb2652930

Size : 100ml

Brand : Biorbyt

Contact local distributor :


Phone : +1 850 650 7790

Eosin Y

SKU: orb2652930

Description

Hematoxylin and Eosin (H&E) staining is used most commonly in histology.

Research Area

Pharmacology & Drug Discovery

Images & Validation

−
Eosin Y
Application Notes
H and E staining: Follow the procedure as outlined for H and E staining. We use the following protocol: 1. Deparafinize formalin-fixed paraffin-embedded (FFPE) tissue sections. 2. Stain with hematoxylin for 4 minutes (2X), wash 4-5X with tap water, and rinse 2 X with de/di water. 3. Dip in 10 % acetic acid 2X, wash with de/di water, wash with Immuno Automation buffer, PBS or any buffer with pH 7.4-8. 4. Rinse 2X with 70% ethanol, apply Eosin stain 1-5 minutes, and wash with 70% ethanol to remove excess Eosin.. 5. Rinse 2X with 95% ethanol, 2X with 100% ethanol, xylene or any dehydrating agent. 6. The slide can be mounted with organic mounting medium or our Organo /limonene mounting medium.

Key Properties

−

Storage & Handling

−
StorageRoom Temperature
Expiration Date12 months from date of receipt.
DisclaimerFor research use only
−
  • Eosin Y

    Eosin Y [orb2652928]

    IHC

    500 ml
  • Eosin Y

    Eosin Y [orb2652929]

    IHC

    250 ml
  • Eosin Y

    Eosin Y [orb2652931]

    IHC

    15 ml
  • AMI5

    AMI5 [orb1306204]

    100 mg, 1 g, 500 mg, 1 ml x 10 mM (in DMSO)

Protocol Information

IHC
Immunohistochemistry
View Protocol