ERC1 siRNA (Rat)
Cat# MBS827174-30nmol
Size : 30nmol
Brand : MyBiosource
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ERC1 siRNA – ELKS/Rab6-Interacting/CAST Family Member 1 (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827174MBS827174 is an siRNA targeting ERC1. Also known as ERC1 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit ERC1 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
ERC1, siRNA
Full Product Name
ERC1 siRNA (Rat)
Product Gene Name
ERC1 sirna
Product Synonym Names
CAST2; ELKS; RAB6IP2; ELKS/Rab6-interacting/CAST family member 1; ERC-1; CAZ-associated structural protein 2; CAST2; Rab6-interacting protein 2
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
ERC1 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
108,818 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat ERC1 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for ERC1 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of ERC1 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for ERC1. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
ELKS/Rab6-interacting/CAST family member 1
NCBI Official Synonym Full Names
ELKS/RAB6-interacting/CAST family member 1
NCBI Official Symbol
Erc1
NCBI Official Synonym Symbols
ERC; CAST; Elks; Cast2; ERC1b; Rab6ip2
UniProt Protein Name
ELKS/Rab6-interacting/CAST family member 1
UniProt Synonym Protein Names
CAZ-associated structural protein 2; CAST2; Rab6-interacting protein 2
Protein Family
ELKS/Rab6-interacting/CAST family
UniProt Gene Name
Erc1
UniProt Synonym Gene Names
Cast2; Elks; Rab6ip2; ERC-1; CAST2
UniProt Entry Name
RB6I2_RAT
NCBI Protein Information
ELKS/Rab6-interacting/CAST family member 1
NCBI Summary for ERC1
bind to the conserved PDZ domain of presynaptic active zone proteins termed RIMs [RGD, Feb 2006]
UniProt Comments for ERC1
ERC1: Regulatory subunit of the IKK complex. Probably recruits IkappaBalpha/NFKBIA to the complex. May be involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. May be involved in vesicle trafficking at the CAZ. May be involved in Rab-6 regulated endosomes to Golgi transport. Part of a complex with CHUK, IKBKB and IKBKG. Interacts with CHUK, IKBKB and IKBKG. The interaction with IKBKG is independent of CHUK and IKBKB. Interacts with NFKBIA. Isoform 4 interacts with PPFIA1, and through its C-terminus with the PDZ domains of RIMS1 and RIMS2. Interacts with ERC2/CAST1. Interacts with the GTB-bound forms of RAB6A isoform 1 and isoform 2 and with RAB6B. The interaction was strongest with RAB6B, followed by RAB6A isoform 2 and weakest with RAB6A isoform 1. Widely expressed. Isoform 2 and isoform 4 are abundantly expressed in brain. Isoform 1 and isoform 3 are predominantly expressed in testis and thyroid, and isoform 1 predominates in other tissues tested. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold
Cellular Component: Golgi membrane; cytoplasm; synapse; IkappaB kinase complex
Molecular Function: protein binding; Rab GTPase binding; PDZ domain binding
Biological Process: protein transport; I-kappaB phosphorylation; retrograde transport, endosome to Golgi
Show more Protein type: Adaptor/scaffold
Cellular Component: Golgi membrane; cytoplasm; synapse; IkappaB kinase complex
Molecular Function: protein binding; Rab GTPase binding; PDZ domain binding
Biological Process: protein transport; I-kappaB phosphorylation; retrograde transport, endosome to Golgi
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with ERC1 sirna
| Reference Product | PubMed Publications |
|---|---|
| Bsn sirna | >6 publications with ERC1 and Bsn |
| Rims1 sirna | >2 publications with ERC1 and Rims1 |
| Ppfia1 sirna | >1 publications with ERC1 and Ppfia1 |
Pathways associated with ERC1 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| NF-kappa B Signaling Pathway antibodies | NF-kappa B Signaling Pathway Diagram |
Diseases associated with ERC1 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Neoplasms, Experimental Antibodies | >3 publications with ERC1 and Neoplasms, Experimental |
| Carcinoma Antibodies | >3 publications with ERC1 and Carcinoma |
| Liver Neoplasms Antibodies | >2 publications with ERC1 and Liver Neoplasms |
| Lung Neoplasms Antibodies | >2 publications with ERC1 and Lung Neoplasms |
| Kidney Diseases Antibodies | >2 publications with ERC1 and Kidney Diseases |
| Necrosis Antibodies | >2 publications with ERC1 and Necrosis |
| Liver Neoplasms, Experimental Antibodies | >1 publications with ERC1 and Liver Neoplasms, Experimental |
| Adenocarcinoma Antibodies | >1 publications with ERC1 and Adenocarcinoma |
| Neoplasm Metastasis Antibodies | >1 publications with ERC1 and Neoplasm Metastasis |
| Inflammation Antibodies | >1 publications with ERC1 and Inflammation |
Organs/Tissues associated with ERC1 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >8 publications with ERC1 and Brain |