FBP2 siRNA (Rat)
Cat# MBS827036-30nmol
Size : 30nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
FBP2 siRNA – Fructose-1,6-Bisphosphatase Isozyme 2 (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS827036MBS827036 is an siRNA targeting FBP2. Also known as FBP2 siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit FBP2 expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
FBP2, siRNA
Full Product Name
FBP2 siRNA (Rat)
Product Gene Name
FBP2 sirna
Product Synonym Names
Fructose-1.6-bisphosphatase isozyme 2; FBPase 2; D-fructose-1.6-bisphosphate 1-phosphohydrolase 2
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
FBP2 siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
36,887 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat FBP2 gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for FBP2 sirna
RNA Interference (RNAi)
Other Notes
Small volumes of FBP2 sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for FBP2. It may not necessarily be applicable to this product.
NCBI Official Full Name
fructose-1,6-bisphosphatase isozyme 2
NCBI Official Synonym Full Names
fructose-1,6-bisphosphatase 2
NCBI Official Symbol
Fbp2
NCBI Official Synonym Symbols
FBPase
UniProt Protein Name
Fructose-1,6-bisphosphatase isozyme 2
UniProt Synonym Protein Names
D-fructose-1,6-bisphosphate 1-phosphohydrolase 2; Muscle FBPase
Protein Family
Agamous-like MADS-box protein
UniProt Gene Name
Fbp2
UniProt Synonym Gene Names
FBPase 2
UniProt Entry Name
F16P2_RAT
NCBI Protein Information
fructose-1,6-bisphosphatase isozyme 2
NCBI Summary for FBP2
last key-enzyme before the synthesis of glucose-6-phosphate of the gluconeogenic pathway [RGD, Feb 2006]
UniProt Comments for FBP2
FBPase 2: a key enzyme of gluconeogenesis that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, a precursor to glucose 6-phosphate. A regulator of glucose synthesis from non-carbohydrates. Two paralogs of this enzyme exists in humans: FBP1 is expressed in the liver and gluconeogenic tissues, and FBP2 in striated muscle. FBP2 is only isozyme expressed in striated muscle and it is widely expressed in cells that are nongluconeogenic. Both forms are inhibited allosterically by AMP, NAD and Ca2+. The muscle form is about 100-fold more sensitive to AMP and NAD, and about 1000-fold more sensitive to inhibition by Ca2+, the major activator of glycolysis in striated muscle. Forms an active complex with aldolase that binds to the sarcomeric Z-line, which is disrupted by elevated calcium levels, blocking glycogen synthesis during exercise. Forms homotetramers that are stabilized in the active state by divalent cations (Mg2+, Mn2+ , Co+2, or Zn2+). Its interaction with aldolase appears to desensitizes it to inhibition by AMP and, partially, by Ca2+. Up to 50% of lactate generated in striated muscle is converted to glycogen.
Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway
Cellular Component: Z disc; cell junction; nucleus
Molecular Function: metal ion binding; fructose-bisphosphatase activity
Biological Process: dephosphorylation; gluconeogenesis
Show more Protein type: Phosphatase (non-protein); Carbohydrate Metabolism - fructose and mannose; EC 3.1.3.11; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - pentose phosphate pathway
Cellular Component: Z disc; cell junction; nucleus
Molecular Function: metal ion binding; fructose-bisphosphatase activity
Biological Process: dephosphorylation; gluconeogenesis
Research Articles on FBP2
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with FBP2 sirna
| Reference Product | PubMed Publications |
|---|---|
| Gpi sirna | >5 publications with FBP2 and Gpi |
| Pfkfb3 sirna | >1 publications with FBP2 and Pfkfb3 |
| Pfkfb1 sirna | >1 publications with FBP2 and Pfkfb1 |
Pathways associated with FBP2 sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| AMPK Signaling Pathway antibodies | AMPK Signaling Pathway Diagram |
| AMPK Signaling Pathway antibodies | AMPK Signaling Pathway Diagram |
| Biosynthesis Of Antibiotics Pathway antibodies | Biosynthesis Of Antibiotics Pathway Diagram |
| Carbon Metabolism Pathway antibodies | Carbon Metabolism Pathway Diagram |
| Carbon Metabolism Pathway antibodies | Carbon Metabolism Pathway Diagram |
| Fructose And Mannose Metabolism Pathway antibodies | Fructose And Mannose Metabolism Pathway Diagram |
| Fructose And Mannose Metabolism Pathway antibodies | Fructose And Mannose Metabolism Pathway Diagram |
| Gluconeogenesis Pathway antibodies | Gluconeogenesis Pathway Diagram |
| Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies | Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram |
| Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway antibodies | Gluconeogenesis, Oxaloacetate => Fructose-6P Pathway Diagram |
Diseases associated with FBP2 sirna
| Disease Name | Pubmed Publications |
|---|---|
| Inflammation Antibodies | >2 publications with FBP2 and Inflammation |
| Disease Models, Animal Antibodies | >1 publications with FBP2 and Disease Models, Animal |
| Kidney Diseases Antibodies | >1 publications with FBP2 and Kidney Diseases |
| Fatty Liver Antibodies | >1 publications with FBP2 and Fatty Liver |
| Necrosis Antibodies | >1 publications with FBP2 and Necrosis |
Organs/Tissues associated with FBP2 sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Connective Tissue Antibodies | >1 publications with FBP2 and Connective Tissue |
| Small Intestine Antibodies | >1 publications with FBP2 and Small Intestine |
| Prostate Antibodies | >1 publications with FBP2 and Prostate |