Reagents and instruments for immunology, cell biology and molecular biology.
 
> Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (APC)

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (APC)

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Cat# 168643-AF-APC-100ul

Size : 100ul

Brand : US Biological

Contact local distributor :


Phone : +1 850 650 7790

168643-AF-APC Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (APC)

Clone Type
Monoclonal
Host
mouse
Source
human
Swiss Prot
P02771 (Human)
Isotype
IgG2a,k
Grade
Purified
Applications
IF IHC
Crossreactivity
Ca Hu Mk Po
Shipping Temp
Blue Ice
Storage Temp
4°C Do Not Freeze

It recognizes an oncofetal glycoprotein with a single chain of 70kDa, which is identified as alpha fetoprotein (AFP). This MAb is highly specific to AFP and shows no cross-reaction with other oncofetal antigens or serum albumin. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin-fixed): Requires HIER using 10mM sodium citrate buffer pH 6.0 for 10-20min followed by cooling at RT for 20min
Optimal dilutions to be determined by the researcher.

Positive Control:
Hep-G2 cells. Fetal liver or hepatocellular carcinoma

Storage and Stability:
May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.

Note: Applications are based on unconjugated antibody.

Applications
Product Type: Mab|Isotype: IgG2a,k|Clone No: C3|Host: mouse|Source: human|Concentration: As Reported |Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).|Purity: Purified by Protein A/G affinity chromatography.|Immunogen: alpha-Fetoprotein purified from serum of a hepatoma patient. Cellular Localization: Cytoplasmic|Specificity: Recognizes human alpha-Fetoprotein. Species Crossreactivity: monkey, canine, and porcine. It does not react with bovine, mouse, and rat.||Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Immunogen
alpha-Fetoprotein purified from serum of a hepatoma patient. Cellular Localization: Cytoplasmic
Form
Supplied as a liquid in PBS, pH 7.2. Labeled with Allophycocyanin (APC).
Purity
Purified by Protein A/G affinity chromatography.
Specificity
Recognizes human alpha-Fetoprotein. Species Crossreactivity: monkey, canine, and porcine. It does not react with bovine, mouse, and rat.
References
1. Yazova AK; Goussev AI; Poltoranina VS; Yakimenko EF. Human alpha-fetoprotein epitopes as revealed by monoclonal antibodies. Immunology Letters, 1990 Sep, 25(4):325-30.