
REAL-TIME PCR INSTRUMENTATION
TSQ-4096 Real-Time PCR SystemFour-channel fluorescence detection for precise, flexible real-time PCR analysis.
The TSQ-4096 Real-Time PCR System combines precise thermal control, LED-based fluorescence excitation and four-channel detection to support quantitative and qualitative nucleic acid analysis.
Designed for molecular biology research, the system supports multiple analytical workflows, including gene expression studies, nucleic acid quantification, melting curve analysis and genotyping.
Product Description
The TSQ-4096 is a real-time PCR instrument designed to amplify nucleic acids while monitoring fluorescence signals throughout the amplification process. Its integrated optical detection system uses LED light sources, optical filters and photodiodes to measure fluorescence from compatible DNA-binding dyes and labeled probes.
The instrument offers four fluorescence channels, enabling assays using commonly employed fluorophores such as FAM, SYBR Green I, HEX, VIC, ROX and Cy5, according to channel compatibility. This flexibility supports a range of single-target and multiplex assay designs.
Precise temperature regulation helps maintain consistent amplification conditions. The instrument incorporates independent temperature control, a thermostatic hot lid and a convenient sample-loading platform.
Its built-in touchscreen allows stand-alone operation, while the accompanying application software supports experiment configuration, real-time monitoring, data analysis and reporting.
Researchers can use the TSQ-4096 for applications such as gene expression analysis, DNA quantification, mutation studies, genotyping and other nucleic acid research workflows.
Technical Specifications
| Sample format | 96-well format |
|---|---|
| Fluorescence channels | 4 |
| Excitation light source | LED |
| Detection system | Photodiodes |
| Temperature accuracy | 0.1°C |
| Temperature uniformity | ±0.1°C |
| Temperature precision | 0.1°C |
| Maximum cooling rate | 5.0°C/s |
| Average cooling rate | 2.8°C/s |
| Display | Built-in 10.4-inch color touchscreen |
| Connectivity | Ethernet (TCP/IP), USB 2.0 |
| Power supply | AC 100–240 V, 50/60 Hz |
| Rated power | 900 VA |
| Operating temperature | 10–30°C |
| Operating humidity | 20–85% RH, non-condensing |
| Maximum operating noise | 65 dB |
Fluorescence Detection Channels
Four optical detection channels support a range of commonly used fluorescent dyes for real-time PCR assays.
| Channel | Compatible dyes listed in manual |
|---|---|
| Channel 1 | FAM, SYBR Green I, LC Green, Eva Green, SYTO 9 |
| Channel 2 | HEX, TET, VIC, JOE |
| Channel 3 | Texas Red, ROX |
| Channel 4 | Cy5 |
Software & Analysis Functions
| Absolute quantification | Quantification of nucleic acids using standard curves. |
|---|---|
| Relative quantification | Comparison of target nucleic acid levels between samples. |
| Melting curve analysis | Evaluation of amplification product melting characteristics. |
| High-resolution melting (HRM) | Analysis of melting profile differences for sequence variation studies. |
| Genotyping | Allelic discrimination using fluorescence-labeled probes. |
| Endpoint fluorescence | Analysis of fluorescence measurements at an endpoint. |
| Experiment storage | Up to 1,000 experiment setting or data files. |
| Network control | A control computer can connect to up to 10 instruments, according to the manual. |
Explore Real-Time PCR Products
Discover our selection of real-time PCR products for gene expression analysis, nucleic acid quantification and fluorescence-based molecular biology applications. Compare available solutions to identify the system best suited to your laboratory requirements.


