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Reagents and instruments for immunology, cell biology and molecular biology.

Goat Rabbit IgG (H&L) Antibody (biotin)

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Cat# orb1236938-2mg

Size : 2mg

Contact local distributor :

Phone : +1 850 650 7790

Brand : Biorbyt

Request more information

Contact local distributor :

Phone : +1 850 650 7790

Goat Rabbit IgG (H&L) Antibody (biotin)

SKU: orb1236938

Description

Goat Rabbit IgG (H&L) Antibody (biotin)

Research Area

Cell Biology

Images & Validation

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Tested Applications	ELISA
Reactivity	Rabbit
Application Notes	Biotin Conjugated Affinity Purified anti-Rabbit IgG is suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assay using streptavidin or avidin conjugates requiring lot-to-lot consistency. This product has been assayed against 1.0 ug of Rabbit IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiozoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:500,000 of the reconstitution concentration is suggested for this product. (Optimal dilution should be determined by user).

Key Properties

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Antibody Type	Secondary Antibody
Host	Goat
Isotype	IgG
Immunogen	Rabbit IgG - whole molecule
Purification	Biotin Conjugated Affinity Purified anti-Rabbit IgG was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Human Serum Proteins.
Conjugation	Biotin

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, 0.01% (w/v) Sodium Azide; pH 7.2; 10 mg/mL Bovine Serum Albumin
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Goat Anti-Rabbit IgG H&L, Biotin conjugated [orb868233]
Applications:
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Reactivity:
Rabbit
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 100 μl, 1 ml
Goat Rabbit IgG (H+L) Secondary Antibody (Biotin) [orb1474935]
Applications:
ELISA, IHC
Reactivity:
Rabbit
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 0.5 ml, 1 ml
F(ab')₂ Fragment Goat Anti-rabbit IgG H&L, Biotin conjugated [orb1974590]
Applications:
ELISA, FC, ICC, IF, IHC-Fr, IHC-P, WB
Reactivity:
Rabbit
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 100 μl
Rabbit IgG (H&L) Secondary Antibody Biotin Conjugated Pre-Adsorbed [orb347656]
Applications:
ELISA, IHC, WB
Reactivity:
Rabbit
Species/Host:
Goat
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 1 mg
Rabbit Anti-Goat IgG (H+L) Secondary Antibody (Biotin) [orb633221]
Applications:
ELISA, ICC, IHC, WB
Reactivity:
Goat
Species/Host:
Rabbit
Clonality:
Polyclonal
Conjugation:
Biotin
Sizes Available: 100 μl, 500 μl

Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)