GRP siRNA (Mouse)
Cat# MBS826976-30nmol
Size : 30nmol
Brand : MyBiosource
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Phone : +1 850 650 7790
GRP siRNA – Gastrin-Releasing Peptide Preproprotein (Mouse)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS826976MBS826976 is an siRNA targeting GRP. Also known as GRP siRNA (Mouse). Reactive in Mouse. Host: Synthetic. siRNA to inhibit GRP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
GRP, siRNA
Full Product Name
GRP siRNA (Mouse)
Product Gene Name
GRP sirna
Product Synonym Names
Gastrin-releasing peptide; GRP
Host
Synthetic
Species Reactivity
Mouse
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
GRP siRNA (Mouse) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
15,659 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of mouse GRP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for GRP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of GRP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for GRP. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
gastrin-releasing peptide preproprotein
NCBI Official Synonym Full Names
gastrin releasing peptide
NCBI Official Symbol
Grp
NCBI Official Synonym Symbols
BLP
UniProt Protein Name
Gastrin-releasing peptide
UniProt Synonym Protein Names
Neuromedin-CAlternative name(s):GRP-10
Protein Family
Beta-1,3-glucan-binding protein
UniProt Gene Name
Grp
UniProt Synonym Gene Names
GRP
UniProt Entry Name
GRP_MOUSE
NCBI Protein Information
gastrin-releasing peptide
NCBI Summary for GRP
This gene encodes a neuropeptide hormone that affects various biological processes such as neuroendocrine regulation, gastrointestinal secretion, nociception, cell proliferation and inflammation. The encoded protein undergoes proteolytic processing to generate multiple mature peptides with biological activity. [provided by RefSeq, Aug 2015]
Show more UniProt Comments for GRP
GRP: GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP- receptor gene expression, long-term potentiation, and amygdala- dependent memory for fear. Belongs to the bombesin/neuromedin-B/ranatensin family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: neuropeptide hormone activity
Biological Process: neuropeptide signaling pathway
Show more Protein type: Secreted; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: neuropeptide hormone activity
Biological Process: neuropeptide signaling pathway
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with GRP sirna
| Reference Product | PubMed Publications |
|---|---|
| Grpr sirna | >145 publications with GRP and Grpr |
| Cck sirna | >111 publications with GRP and Cck |
| Nmbr sirna | >24 publications with GRP and Nmbr |
| Nts sirna | >4 publications with GRP and Nts |
Pathways associated with GRP sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Class A/1 (Rhodopsin-like Receptors) Pathway antibodies | Class A/1 (Rhodopsin-like Receptors) Pathway Diagram |
| G Alpha (q) Signalling Events Pathway antibodies | G Alpha (q) Signalling Events Pathway Diagram |
| GPCR Downstream Signaling Pathway antibodies | GPCR Downstream Signaling Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| Gastrin-CREB Signalling Pathway Via PKC And MAPK antibodies | Gastrin-CREB Signalling Pathway Via PKC And MAPK Diagram |
| Incretin Synthesis, Secretion, And Inactivation Pathway antibodies | Incretin Synthesis, Secretion, And Inactivation Pathway Diagram |
| Metabolism Of Proteins Pathway antibodies | Metabolism Of Proteins Pathway Diagram |
| Peptide Hormone Metabolism Pathway antibodies | Peptide Hormone Metabolism Pathway Diagram |
| Peptide Ligand-binding Receptors Pathway antibodies | Peptide Ligand-binding Receptors Pathway Diagram |
| Signal Transduction Pathway antibodies | Signal Transduction Pathway Diagram |
Diseases associated with GRP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Lung Neoplasms Antibodies | >228 publications with GRP and Lung Neoplasms |
| Lung Diseases Antibodies | >219 publications with GRP and Lung Diseases |
| Inflammation Antibodies | >128 publications with GRP and Inflammation |
| Breast Neoplasms Antibodies | >87 publications with GRP and Breast Neoplasms |
| Skin Diseases Antibodies | >80 publications with GRP and Skin Diseases |
| Fibrosis Antibodies | >59 publications with GRP and Fibrosis |
| Necrosis Antibodies | >58 publications with GRP and Necrosis |
| Kidney Diseases Antibodies | >56 publications with GRP and Kidney Diseases |
| Neoplasm Metastasis Antibodies | >39 publications with GRP and Neoplasm Metastasis |
| Head and Neck Neoplasms Antibodies | >39 publications with GRP and Head and Neck Neoplasms |
Organs/Tissues associated with GRP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Other Antibodies | >582 publications with GRP and Other |
| Brain Antibodies | >419 publications with GRP and Brain |
| Stomach Antibodies | >189 publications with GRP and Stomach |
| Intestine Antibodies | >170 publications with GRP and Intestine |
| Spinal Cord Antibodies | >97 publications with GRP and Spinal Cord |
| Embryonic Tissue Antibodies | >54 publications with GRP and Embryonic Tissue |
| Thyroid Antibodies | >41 publications with GRP and Thyroid |
| Eye Antibodies | >35 publications with GRP and Eye |
| Spleen Antibodies | >17 publications with GRP and Spleen |