The Hantaan Orthohantavirus Nucleocapsid (N) Human IgA ELISA Kit is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against HTNVN antigen(s) in human serum or plasma.
Hantaan Orthohantavirus Nucleocapsid (N) Human IgA ELISA Kit Standard Curve
Hantaan Orthohantavirus Nucleocapsid (N) Human IgA ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Hantaan Orthohantavirus |
| Detection Range | 1.37 Units/mL - 1000 Units/mL |
| Compatible Sample Types | Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 1,000-fold |
| Gene Symbols | IgA |
| Protein Name / Synonyms | IgA; Hantaan Orthohantavirus Nucleocapsid (N) |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Indirect |
| Method Of Detection | Colorimetric |
| Research Area | Infectious Disease, Hemorrhagic Fever |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
Introduction
The Hantaan Orthohantavirus Nucleocapsid (N) Human IgA ELISA Kit employs an indirect ELISA method. In this kit, standard 96-well plates (12 strips with 8 wells/strip) are coated with HTNVN protein, which combines with the corresponding antibody present in a sample and Positive Control, which is used as a calibration curve for interpretation purposes. When a biotinylated secondary anti-human IgA antibody is added, a complex forms between biotinylated secondary antibody and human antibody bound to the protein coated plate. A TMB substrate is added, and a blue color is generated. The depth of color is relative to the amount of the human anti-HTNVN antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Kit Components
| Component | Size / Description |
|---|---|
| HTNVN Coated 96-Well Microplate | 96 wells coated with HTNVN |
| Wash Buffer | 25 mL |
| Positive Control | 1 vial |
| Biotinylated Anti-Human IgA | 1 vial |
| HRP-Streptavidin | 1 vial |
| TMB One-Step Substrate Reagent | 12 mL |
| Stop Solution | 8 mL |
| Assay Diluent | 15 mL |
| Sample Diluent | 25 mL |
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 100 µL positive control, or sample to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Biotinylated Anti-Human IgA Antibody into respective wells. Incubate 1 hour at room temperature.
- Add 100 µL prepared HRP-Streptavidin solution to each well. Incubate 30 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 10 to 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The unopened kit may be stored at -20°C to -80°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles.