Histone H1.3, phosphorylated (Thr17) +H1.4, phosphorylated (Thr17)

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence/Immunocytochemistry, and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:3000, incubate membrane with diluted primary antibody in TBS, 5% milk, 0.1% Tween 20 at 4°C with gentle shaking,
overnight.
Immunohistochemistry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.