Flow Cytometry
Surface staining of human peripheral blood cells with HLA-A monoclonal antibody, clone W6/32 (Cat # MAB6449) Alexa Fluor® 488.
- Specifications
Product Description
Mouse monoclonal antibody raised against native HLA-A.
Immunogen
Native purified HLA-A from membrane of human tonsil cells.
Host
Mouse
Reactivity
Bovine, Cat, Human, Non-Human Primates
Specificity
This antibody recognizes MHC Class I molecules (MHC Class Ia) that are expressedon the surface of all human nucleated cell types. This antibody is also suitable as a positive control for HLA tissue typing andcrossmatching.
Form
Liquid
Conjugation
FITC
Concentration
1 mg/mL
Isotype
IgG2a
Recommend Usage
The optimal working dilution should be determined by the end user.
Storage Buffer
In PBS, pH 7.4
Storage Instruction
Store at 4°C. Do not freeze.
- Applications
Functional Study
Immunohistochemistry (Frozen sections)
Flow Cytometry
Surface staining of human peripheral blood cells with HLA-A monoclonal antibody, clone W6/32 (Cat # MAB6449) Alexa Fluor® 488.Enzyme-linked Immunoabsorbent Assay
Immunoprecipitation
Western Blot
- Gene Info — HLA-A
Entrez GeneID
3105Gene Name
HLA-A
Gene Alias
HLAA
Gene Description
major histocompatibility complex, class I, A
Gene Ontology
HyperlinkGene Summary
HLA-A belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-A alleles have been described. [provided by RefSeq
Other Designations
HLA class I histocompatibility antigen, A-23 alpha chain|MHC class I antigen HLA-A heavy chain|MHC leukocyte antigen|OTTHUMP00000161059|antigen presenting molecule|leucocyte antigen class I|leukocyte antigen class I-A
- Interactomes
- Pathways
- Diseases