HSV 2 (strain 333) Glycoprotein B/gB Protein (His)

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Cat# TMPY-06816-20ug

Size : 20ug

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Phone : +1 850 650 7790

HSV 2 (strain 333) Glycoprotein B/gB Protein (His)

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HSV 2 (strain 333) Glycoprotein B/gB Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 73.88 kDa and the accession number is P06763.

For research use only—not for human use. No sales to individuals. Use as intended only.

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User Guide of Recombinant Proteins

Product Introduction

Bioactivity

Bioactivity	Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description	HSV 2 (strain 333) Glycoprotein B/gB Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 73.88 kDa and the accession number is P06763.
Species	HSV2
Expression System	Baculovirus Insect Cells
Tag	C-His
Accession Number	P06763
Construction	A DNA sequence encoding the HSV 2 (strain 333 (HSV2)) gB (translated amino acids of P06763) (Glu98-Ala730) was expressed with a polyhistidine tag at the C-terminus. Predicted N terminal: Glu 98
Protein Purity	≥ 95 % as determined by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein as determined by the LAL method.
Formulation	Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM Tris, 200 mM NaCl, 20% glycerol, pH 8.0.Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
Reconstitution	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Research Background	Cytomegalovirus (CMV) (human herpesvirus 5) glycoprotein B, also referred as CMV gB or gB, which belongs to the herpesviridae glycoprotein B family. It is a 97-amino acid glycoprotein encoded by the ORF of UL55. Cytomegalovirus Glycoprotein B protein is the most abundant component of the envelope, a target of neutralizing antibodies with at least two defined neutralizing epitopes and an essential replication component. Cytomegalovirus Glycoprotein B protein plays important roles in HCMV entry, cell-cell spread of internal virions, and fusion of infected cells. In addition, Cytomegalovirus Glycoprotein B protein is one envelope protein capable of heparin binding. It forms a physical association with host cell annexin II independent of the presence of calcium.

Chemical Properties

Molecular Weight

73.88 kDa (predicted); 71.01 kDa (reducing conditions)

Storage & Solubility Information

Shipping	In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
Storage	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.