Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to HNE. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated HNE. The competitive inhibition reaction is launched between with HRP-conjugated HNE and HNE in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of HNE in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of HNE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS706111 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the 4-Hydroxynonenal (HNE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HNE. The ELISA analytical biochemical technique of the MBS706111 kit is based on HNE antibody-HNE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HNE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HNE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).