Quantitative, sandwich-based, glass slide antibody array to detect 10 human angiogenic factors. Suitable for all liquid sample types.
Human Angiogenesis Array Q1
Human Angiogenesis Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 10 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | Angiogenesis, HER/ErbB Signaling, Vascular Biology |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
Angiogenin
| Angiopoietin-2
| EGF
| bFGF
| HB-EGF
|
HGF
| Leptin
| PDGF-BB
| PLGF
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Angiogenesis Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-ANG-1.gal)
Excel (QAH-ANG-1_Analysis_Tool.xls)
citations
Imaging of Angiogenesis in White Matter Hyperintensities
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Journal of the American Heart Association
MenSCs Transplantation Improve the Viability of Injured Endometrial Cells Through Activating PI3K/Akt Pathway
- Species:
- Human
- Sample Type:
- menstrual blood-derived endometrial stem cells (MenSCs)
- Year:
- 2023
- Journal Name:
- Reproductive Sciences
BZD9L1 benzimidazole analogue hampers colorectal tumor progression by impeding angiogenesis
- Species:
- Human
- Sample Type:
- Ea. hy926 cell
- Year:
- 2023
- Journal Name:
- World Journal of Gastrointestinal Oncology
Vascular toxicity of multi-walled carbon nanotubes targeting vascular endothelial growth factor
- Species:
- Human
- Sample Type:
- human umbilical vein endothelial cell (HUVECs)
- Year:
- 2022
- Journal Name:
- Nanotoxicology
Sustainable Production and Activity Determination of Serum-Free Conditioned Medium from Menstrual Blood-Derived Endometrial Stem Cells
- Species:
- Human
- Sample Type:
- menstrual blood-derived endometrial stem cells (MenSCs)
- Year:
- 2022
- Journal Name:
- Applied Biochemistry and Biotechnology
Modulation of the in vitro angiogenic potential of human mesenchymal stromal cells from different tissue sources
- Species:
- Human
- Sample Type:
- Conditioned media (UCM MSC culture media)
- Year:
- 2020
- Journal Name:
- Journal of Cellular Physiology
Synergistic anti-hepatoma effect of bufalin combined with sorafenib via mediating the tumor vascular microenvironment by targeting mTOR/VEGF signaling
- Species:
- Mouse
- Sample Type:
- Conditioned Media (HCC cells)
- Year:
- 2018
- Journal Name:
- International Journal of Oncology
Angiogenesis in Liquid Tumors: An In Vitro Assay for Leukemic Cell Induced Bone Marrow Angiogenesis.
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2016
- Journal Name:
- Advances in Healthcare Materials
- PubMed ID:
- 26924785
Alginate microencapsulation of human mesenchymal stem cells as a strategy to enhance paracrine-mediated vascular recovery after hindlimb ischaemia
- Species:
- Human
- Sample Type:
- Extracellular Vesicles (Capsules from MSCs contatining cytokines)
- Year:
- 2016
- PubMed ID:
- 23281223
The Effect of Living Cellular Sheets on the Angiogenic Potential of Human Microvascular Endothelial Cells
- Species:
- Human
- Sample Type:
- Conditioned Media (Human Microvascular Endothelial Cells Culture Sup)
- Year:
- 2015
- Journal Name:
- Journal of Periodontology