RayBio® Human bFGF ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.
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Human bFGF ELISA Kit
Human bFGF ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human |
| Sensitivity | 50 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 50 pg/ml - 10000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P09038 |
| Gene Id | 2247 |
| Gene Symbols | FGF2, FGFB |
| Protein Name / Synonyms | Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Angiogenesis |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
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Target Background
Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, is a member of a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. Additional members of the FGF family include the oncogenes FGF-3 (Int2) and FGF-4 (HST/Kaposi), FGF-5, FGF-6, FGF-7 (KGF), FGF-8 (AIGF), FGF-9 (GAF) and FGF-10. Members of the FGF family share 30-55% amino acid sequence identity and similar gene structure, and are capable of transforming cultured cells when overexpressed in transfected cells. Cellular receptors for FGFs are members of a second multigene family including four tyrosine kinases, designated Flg (FGFR-1), Bek (FGFR-L), TKF and FGFR-3
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
documents
Excel (Sandwich-ELISA-Analysis-Tool.xlsm)
citations
Braided suture-reinforced fibrous yarn bundles as a scaffold for tendon tissue engineering in extensor digitorum tendon repair
- Species:
- Rabbit
- Sample Type:
- rabbit tendon-derived fibroblasts (rTFs)
- Year:
- 2022
- Journal Name:
- Chemical Engineering Journal
Autocrine TGF-alpha is associated with Benzo(a)pyrene-induced mucus production and MUC5AC expression during allergic asthma
- Species:
- Human
- Sample Type:
- NCI-H292 cell
- Year:
- 2022
- Journal Name:
- Ecotoxicology and Environmental Safety
Proteomic aptamer analysis reveals serum markers that characterize preclinical systemic sclerosis (SSc) patients at risk for progression toward definite SSc
- Species:
- Human
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Arthritis Research & Therapy
3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering.
- Species:
- Human
- Sample Type:
- Plasma (prp)
- Year:
- 2019
- Journal Name:
- Bioactive materials
Transdermal delivery of bFGF with sonophoresis facilitated by chitosan nanocarriers
- Species:
- Mouse
- Sample Type:
- skin tissue
- Year:
- 2022
- Journal Name:
- Journal of Drug Delivery Science and Technology
Networked lymphatic endothelial cells in a transplanted cell sheet contribute to form functional lymphatic vessels
- Species:
- Human
- Sample Type:
- adipose-derived stem cells (ASCs)
- Year:
- 2022
- Journal Name:
- Scientific reports
In vitro and ex vivo characterization of nanonized amniotic membrane particles: An untapped modality for ocular surface reconstruction
- Species:
- Human
- Sample Type:
- Amniotic Membrane (AM)
- Year:
- 2023
- Journal Name:
- Experimental Eye Research
Tailor Design Electrospun Composite Nanofibers with Staged Release of Multiple Angiogenic Growth Factors for Chronic Wound Healing
- Species:
- Human
- Sample Type:
- Nanomaterials (Nanomaterials incorporation of bFGF)
- Year:
- 2014
- Journal Name:
- Acta Biomaterialia
Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.
- Species:
- Human
- Sample Type:
- Conditioned Media (PAm6-co-A2AGA2 and PAm6-co-PSS2 hydrogels)
- Year:
- 2013
- Journal Name:
- Biomaterials
- PubMed ID:
- 23131532
Human Sertoli cells support high levels of Zika virus replication and persistence
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2018
- Journal Name:
- Scientific reports
- PubMed ID:
- 29615760
