Human CRP ELISA Kit

Cat# ELH-CRP-5

Size : 5PlateKit

Brand : RayBiotech

Contact local distributor :


Phone : +1 850 650 7790

Human CRP ELISA Kit

RayBio® Human CRP (C-Reactive Protein) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Contact Us for Bulk Request

Product Description

Specifications

Size1 Plate Kit, 2 Plate Kit, 5 Plate Kit
SpeciesHuman
Sensitivity
34 pg/ml

Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology.

Detection Range
34 pg/ml - 25,000 pg/ml
Specificity
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, GM-CSF, Leptin (OB), MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, MMP-1, -2, -3, -10, PARC, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Compatible Sample TypesCell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)
20,000 fold
Accession Number
P02741
Gene Id
1401
Gene Symbols
CRP, PTX1
Protein Name / Synonyms
C-reactive protein [Cleaved into: C-reactive protein(1-205)]
Quantitative/Semi-QuantitativeQuantitative
Solid Support
96-well Microplate
Design PrincipleSandwich-based
Method Of DetectionColorimetric
Research AreaCardiovascular Disease, Inflammation
Shipping TypeBlue ice
Storage≤-20°C

Target Background

C-reactive protein (CRP) belongs to the pentaxin family. It is involved in several host defense related functions based on its ability to recognize foreign pathogens and damaged cells of the host and to initiate their elimination by interacting with humoral and cellular effector systems in the blood. CRP is synthesized by the liver in response to factors released by fat cells (adipocytes).Consequently, the level of this protein in plasma increases greatly during acute phase response to tissue injury, infection, or other inflammatory stimuli. CRP is used mainly as a marker of inflammation. Apart from liver failure, there are few known factors that interfere with CRP production. Measuring and charting CRP values can prove useful in determining disease progress or the effectiveness of treatments. Blood, usually collected in a serum-separating tube, is analysed in a medical laboratory or at the point of care.

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Human CRP ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

  • Post-translational modifications (PTMs): Detect modified CRP (C-Reactive Protein), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
  • Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
  • Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

documents

Excel (Sandwich-ELISA-Analysis-Tool.xlsm)

citations

  • Associations of growth differentiation factor 15 with kidney function in frail and pre-frail older individuals: the mediating role of inflammation and oxidative stress

    Species:
    Human
    Sample Type:
    Plasma (frail and pre-frail older individuals)
    Year:
    2025
    Journal Name:
    European journal of medical research
    PubMed ID:
    41345886
  • Cross-sectional association between brain-derived neurotrophic factor and intrinsic capacity in older adults: The mediating role of oxidative stress

    Species:
    Human
    Sample Type:
    Plasma (community-dwelling older adults)
    Year:
    2025
    Journal Name:
    The journal of nutrition, health & aging
    PubMed ID:
    40513379
  • Evaluating Pro- and Anti-inflammatory Biomarkers for Predicting Type 2 Diabetes Mellitus in the Geriatric Population and Correlation With Clinical and Biochemical Parameters

    Species:
    Human
    Sample Type:
    Serum (geriatric subjects, fasting)
    Year:
    2025
    Journal Name:
    Curēus
    PubMed ID:
    39991369
  • Lipocalin-2 Serum Levels in Rheumatoid Arthritis Patients Treated with Adalimumab and Its Correlation with Proinflammatory Factors

    Species:
    Human
    Sample Type:
    Serum (healthy, OA and RA patients)
    Year:
    2024
    Journal Name:
    Mediators of Inflammation
    PubMed ID:
    39403549
  • Pilot Study of IL-1 Antagonist Anakinra for Treatment of Endometriosis

    Species:
    Human
    Sample Type:
    Plasma (endometriosis patients, anakinra vs placebo)
    Year:
    2024
    Journal Name:
    International journal of women's health
    PubMed ID:
    39355382
  • Immunoaging at Early Ages Could Drive a Higher Comorbidity Burden in People with HIV on Antiretroviral Therapy Compared with the Uninfected Population

    Species:
    Human
    Sample Type:
    Plasma (people with HIV on ART)
    Year:
    2024
    Journal Name:
    International Journal of Molecular Sciences
    PubMed ID:
    39456715
  • Prevalence of Anti-Anisakis simplex Antibodies in a Cohort of Patients with Inflammatory Bowel Disease in Norway

    Species:
    Human
    Sample Type:
    Plasma (IBD patients)
    Year:
    2025
    Journal Name:
    Pathogens
    PubMed ID:
    40872279
  • Trough levels of ipilimumab in serum as a potential biomarker of clinical outcomes for patients with advanced melanoma after treatment with ipilimumab

    Species:
    Human
    Sample Type:
    Serum (advanced melanoma, ipilimumab)
    Year:
    2021
    Journal Name:
    Journal for Immunotherapy of Cancer
    PubMed ID:
    34620702
  • Probiotic effects on immunity and microbiome in HIV-1 discordant patients

    Species:
    Human
    Sample Type:
    Plasma
    Year:
    2022
    Journal Name:
    Frontiers in immunology
  • Adipokines and Inflammatory Markers in Acute Myocardial Infarction Patients with and without Obstructive Sleep Apnea: A Comparative Analysis

    Species:
    Human
    Sample Type:
    Serum
    Year:
    2023
    Journal Name:
    International Journal of Molecular Sciences

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