RayBio® C-Series Human Cytokine Antibody Array 1 Kit. Detects 23 Humans Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C1
Human Cytokine Array C1
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 23 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
| GCSF
| GM-CSF
| GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| IL-1 alpha (IL-1 F1)
|
| IL-2
| IL-3
| IL-5
| IL-6
| IL-7
|
| IL-8 (CXCL8)
| IL-10
| IL-13
| IL-15
| IFN-gamma
|
| MCP-1 (CCL2)
| MCP-2 (CCL8)
| MCP-3 (MARC/CCL7)
| MIG (CXCL9)
| RANTES (CCL5)
|
| TGF beta 1
| TNF alpha
| TNF beta (TNFSF1B)
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C1 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
citations
Tumor-derived exosomes modulate PD-L1 expression in monocytes.
- Species:
- human
- Sample Type:
- Serum
- Year:
- 2017
- Journal Name:
- Science Immunology
EPAS1 prevents telomeric damage-induced senescence by enhancing transcription of TRF1, TRF2, and RAD50
- Species:
- Mouse
- Sample Type:
- fibroblasts
- Year:
- 2023
- Journal Name:
- Zoological research
Suppression of the antitumoral activity of natural killer cells under indirect coculture with cancer-associated fibroblasts in a pancreatic TIME-on-chip model
- Species:
- Human
- Sample Type:
- PANC-1 tumor spheroids (TSs) (NK-92 cell, activated pancreatic stellate cells (aPSCs))
- Year:
- 2023
- Journal Name:
- Cancer Cell International
Molecular basis for nuclear accumulation and targeting of the inhibitor of apoptosis BIRC2
- Species:
- Human
- Sample Type:
- Human peripheral blood monocytes
- Year:
- 2023
- Journal Name:
- Nature Structural & Molecular Biology
Evaluation of the Immunomodulatory Effects of a Probiotics and Natural Extract-Based Formulation in Bacterial-Induced Prostatitis
- Species:
- Human
- Sample Type:
- LNCaP cell
- Year:
- 2023
- Journal Name:
- Life-Basel
Contactless Resolution of Inflammatory Signals in Tailored Macrophage-Based Cell Therapeutics
- Species:
- Human
- Sample Type:
- THP1 cell
- Year:
- 2023
- Journal Name:
- Acs Applied Materials & Interfaces
Topoisomerase 1 cleavage complex enables pattern recognition and inflammation during senescence
- Species:
- Human
- Sample Type:
- Conditioned media (Cell culture media)
- Year:
- 2020
- Journal Name:
- Nature Communications
Leukemia-on-a-chip: Dissecting the chemoresistance mechanisms in B cell acute lymphoblastic leukemia bone marrow niche
- Species:
- Human
- Sample Type:
- Conditioned Media
- Year:
- 2020
- Journal Name:
- Science advances
Cytoplasmic chromatin triggers inflammation in senescence and cancer
- Species:
- Human
- Sample Type:
- Conditioned Media (IMR90 senescent cell model)
- Year:
- 2017
- Journal Name:
- Nature
Wnt5A exerts immunomodulatory activity in the human ovarian cancer cell line SKOV-3
- Species:
- Human
- Sample Type:
- Conditioned Media (Wnt5a siRNA transfected SKOV-3 cells, an ovarian cancer cell line)
- Year:
- 2016
- Journal Name:
- Cell Biology International
- PubMed ID:
- 26462870