RayBio® C-Series Human Cytokine Antibody Array 4 Kit. Detects 36 Human Cytokines. Suitable for all liquid sample types.
Human Cytokine Array C4
Human Cytokine Array C4
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 36 |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
BDNF
| BLC (CXCL13)
| CK beta 8-1 (CCL23)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
Eotaxin-3 (CCL26)
| FGF-4
| FGF-6
| FGF-7 (KGF)
| FGF-9
|
Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
| GDNF
| HGF
|
IGFBP-1
| IGFBP-2
| IGFBP-3
| IGFBP-4
| IL-16
|
LIF
| LIGHT (TNFSF14)
| MCP-4 (CCL13)
| MIF
| NAP-2 (PPBP/CXCL7)
|
NT-3
| NT-4
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
| PLGF
|
TGF beta 2
| TGF beta 3
| TIMP-1
| TIMP-2
| MIP-3 alpha (CCL20)
|
IP-10 (CXCL10)
| ||||
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C4 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
citations
Reciprocal Signaling between Glioblastoma Stem Cells and Differentiated Tumor Cells Promotes Malignant Progression
- Species:
- Human
- Sample Type:
- Conditioned Media (Glioblastoma stem cells and differentiated cells)
- Year:
- 2018
- Journal Name:
- Cell Stem Cell
- PubMed ID:
- 29625067
Assessment of Some Tools for the Characterization of the Human Osteoarthritic Cartilage Proteome
- Species:
- Human
- Sample Type:
- Conditioned Media (Cartilage tissue-cultured medium)
- Year:
- 2005
- Journal Name:
- Journal of biomolecular techniques
S100A11, a Dual Mediator for Growth Regulation of Human Keratinocytes
- Species:
- human
- Sample Type:
- Cell Lysate (normal human keratinocytes extract)
- Year:
- 2008
- Journal Name:
- Molecular Biology of The Cell
Intracerebroventricular administration of human umbilical cord blood cells delays disease progression in two murine models of motor neuron degeneration.
- Species:
- Human
- Sample Type:
- Conditioned Media (HuCB-MNCs)
- Year:
- 2011
- Journal Name:
- Rejuvenation Research
- PubMed ID:
- 21978082