Quantibody® Human Cytokine Array 1 Kit. Detects 20 Human Cytokines. Suitable for all liquid sample types.
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Human Cytokine Array Q1
Human Cytokine Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Human |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 20 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
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Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
GM-CSF
| GRO alpha/beta/gamma
| IFN-gamma
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
|
IL-10
| IL-12 p70
| IL-13
| IL-2
| IL-4
|
IL-5
| IL-6
| IL-8 (CXCL8)
| MCP-1 (CCL2)
| MIP-1 alpha (CCL3)
|
MIP-1 beta (CCL4)
| MMP-9
| RANTES (CCL5)
| TNF alpha
| VEGF-A
|
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Human Cytokine Array Q1 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAH-CYT-1.gal)
Excel (QAH-CYT-1_Analysis_Tool.xls)
citations
Longevity-Associated Transcription Factor ATF7 Promotes Healthspan by Suppressing Cellular Senescence and Systematic Inflammation
- Species:
- Human
- Sample Type:
- Human dermal fibroblasts (HDFs)
- Year:
- 2023
- Journal Name:
- Aging and disease
Amyloid Beta Peptides Lead to Mast Cell Activation in a Novel 3D Hydrogel Model
- Species:
- Mouse
- Sample Type:
- mast cells (MCs)
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
Botox for the prevention of radiation-induced Sialadenitis and xerostomia in head and neck cancer patients: A pilot study
- Species:
- Human
- Sample Type:
- saliva
- Year:
- 2023
- Journal Name:
- HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK
SARS-CoV-2 Brain Regional Detection, Histopathology, Gene Expression, and Immunomodulatory Changes in Decedents with COVID-19
- Species:
- Human
- Sample Type:
- Serum, cerebrospinal fluid (CSF)
- Year:
- 2022
- Journal Name:
- Journal of Neuropathology and Experimental Neurology
Decreased levels of cytokines implicate altered immune response in plasma of moderate-stage Alzheimer’s disease patients
- Species:
- Human
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Neuroscience Letters
Chronic Kidney Disease Induces Inflammatory CD40+ Monocyte Differentiation via Homocysteine Elevation and DNA Hypomethylation
- Species:
- Human
- Sample Type:
- Serum (Cardiovascular Disease and Kidney Disease Patients)
- Year:
- 2016
- Journal Name:
- Circulation Research
- PubMed ID:
- 27992360
Profiling the human immune response to Mycobacterium tuberculosis by human cytokine array
- Species:
- Human
- Sample Type:
- Serum (Tuberculosis patients)
- Year:
- 2016
Upregulated TCR improves cytokine secretion in T cells from patients with AML
- Species:
- Human
- Sample Type:
- Conditioned Media (TCR zzeta forced expreession in AML T cell patients.)
- Year:
- 2015
- Journal Name:
- Journal of Hematology & Oncology
High-dose dexamethasone modulates serum cytokine profile in patients with primary immune thrombocytopenia
- Species:
- Human
- Sample Type:
- Serum (Human Serum)
- Year:
- 2014
- Journal Name:
- Immunology Letters
Upregulation of innate immune responses in a T cell/histiocyte-rich large B cell lymphoma patient with significant autoimmune disorders mimicking systemic lupus erythematosus
- Species:
- Human
- Sample Type:
- Plasma (R-CHOP treated samples)
- Year:
- 2014
- Journal Name:
- Annals of Hematology