Human epstein-barr virus IgM, EBv IgM ELISA Kit

Intended Uses: For the qualitative determination of activated Human Epstein-Barr Virus Antibody IgM (EBV-IgM) in serum, plasma, tissue homogenate, cell culture supernates and other biological fluids.

Principle of the Assay||The kit uses a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to analyze the existence or not of Human EBV-IgM in samples. Add sample to wells pre-coated with one Human Epstein-Barr virus IgM antigen, at same time add HRP-conjugated Human Epstein-Barr virus IgM antigen to bind the analyte, followed by incubation and washing procedures to remove unbound substance. Finally, HRP substrates are added, incubated for detection, and a blue color is developed. Reaction is stopped and color turns to yellow when Stopping Solution (acidic) is added. The existence or not of Human EBV-IgM in the samples is then determined by comparing the O.D. of the samples to the CUT OFF.

Small volumes of EBv IgM elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9302546 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the epstein-barr virus IgM, EBv IgM, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing EBv IgM. The ELISA analytical biochemical technique of the MBS9302546 kit is based on EBv IgM antibody-EBv IgM antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect EBv IgM antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, EBv IgM. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).