Human GM-CSF Antibody : LE/AF
Cat# OASB01201
Size : 0.5mg
Brand : Aviva Systems Biology
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Phone : +1 850 650 7790
Human GM-CSF Antibody : LE/AF (OASB01201)
| Datasheets/Manuals | Printable datasheet for Human GM-CSF Antibody : LE/AF (OASB01201) |
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| Tested Species Reactivity | Human |
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| Predicted Species Reactivity | Human |
| Clonality | Monoclonal |
| Clone | BVD2-23B6 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | ELISPOT, IP, MP |
| Reconstitution and Storage | Store at -20C |
| Immunogen | E. coli-expressed human GM-CSF |
| Concentration | 0.5 mg/mL |
| Specificity | GM-CSF |
| Characterization | To ensure acceptable performance, each batch of product is tested in a sandwich ELISA to conform tocharacteristics of a standard reference reagent. |
| Application Info | ELISA Capture: Purified BVD2-23B6 monoclonal antibody is the preferred capture antibody in a sandwich ELISA for quantifying human GM-CSF protein levels. 1-4 Purified BVD2-23B6 antibody should be paired with biotinylated BVD2-21C11 antibody (OASB01203) as the detection antibody, with purified recombinant human GM-CSF as the standard. Immunoprecipitation / Western Blotting: The purified BVD2-23B6 antibody is also useful for immunoprecipitation and Western blotting applications. For Western blotting, a concentration of 1-5 ug/mL (in conjunction with AP-labeled Goat Anti-Rat-Ig, OASB01897) has been found to enable detection of <= 100 ng/lane of recombinant human GM-CSF under reducing conditions. Neutralization: The LE/AF form of BVD2-23B6 can be used for neutralizing human GM-CSF bioactivity. A suitable LE/AF Rat IgG2a (OASB00107) should be used as an isotype control. Since applications vary, each investigator should determine the optimal concentration appropriate for individual applications. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Bacchetta, R., T. de Wall Malefyt, H. Yssel, J.S. Abrams, J.E. de Vries, H. Spits, and M.G. Roncarolo. 1990. J. Immunol. 144:902 2. Kita, H., T. Ohnishi, Y. Okubo, D. Weller, J.S. Abrams, and G.J. Gleich. 1991. J. Exp. Med. 174:745 3. Abrams, J.S., M.- G. Roncarolo, H.. Yssel, U. Andersson, G.J. Gleich, and J.E., Silver. 1992. Immunol. Rev. 127:5 4. Abrams, J., J. Silver, R. van Dyke, and G. Gleich. 1994. Eosinophil- active cytokines in human disease: Development and use ofmonoclonal antibodies to IL- 3- , IL- 5, GM- CSF. In Eosinophils in Allergy and Inflammation (A. Kay and G. Gleich, eds). Marcel Dekker,New York, pp. 133. |
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| Gene Symbol | CSF2 |
| Gene Full Name | Granulocyte-macrophage colony-stimulating factor |
| Alias Symbols | CSF, GMCSF |
| NCBI Gene Id | 1437; 574371; 102118747 |
| Protein Name | granulocyte-macrophage colony-stimulating factor |
| Description of Target | The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13. |
| Uniprot ID | P04141, Q9GL44, G7P878 |
| Protein Accession # | NP_000749.2 |
| Nucleotide Accession # | NM_000758.3 |
